Introgression of the Triticum timopheevii Genome Into Wheat Detected by Chromosome-Specific Kompetitive Allele Specific PCR Markers.
Introgression of the Triticum timopheevii Genome Into Wheat Detected by Chromosome-Specific Kompetitive Allele Specific PCR Markers.
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DOI：
10.3389/fpls.2022.919519
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发表时间：
2022
影响因子：
5.6
通讯作者：
King, Ian Phillip
中科院分区：
文献类型：
--
作者：
King, Julie;Grewal, Surbhi;Othmeni, Manel;Coombes, Benedict;Yang, Cai-yun;Walter, Nicola;Ashling, Stephen;Scholefield, Duncan;Walker, Jack;Hubbart-Edwards, Stella;Hall, Anthony;King, Ian Phillip
梯莫菲维小麦（Triticum timopheevii，2n = 28，AtAtGG）是一个四倍体野生近缘种，具有增加六倍体小麦（Triticum aestivum，2n = 42，AABBDD）在许多重要农艺性状上遗传多样性的潜力。小麦-T. timopheevii渐渗系通过进一步的回交和自花受精产生99个渐渗系（IL），其携带来自T. timopheevii。这些基因渗入分别含有来自At和G亚基因组的89和74个独特片段。这些重叠节段覆盖了98.9%的T。timopheevii基因组，现已渗入面包小麦cv. Paragon包括所有T. timopheevii染色体通过不同大小的片段，除了染色体3At，4G和6 G。纯合IL含有1至8个这些基因渗入，平均每个基因渗入系3个。这些纯合基因渗入是通过开发一组480个染色体特异性竞争等位基因特异性PCR（KASP）标记来检测的，这些标记在小麦基因组中分布良好。其中，149个是基于T. timopheevii。这些KASP标记中的大多数也被发现是T。timopheevii亚基因组特异性，182个检测At亚基因组和275个检测G亚基因组片段。这些标记表明98%的At片段与小麦的A基因组重组，74%的G基因组片段与小麦的B基因组重组，其余与小麦的D基因组重组。这些结果通过多色原位杂交分析进行了验证。这些纯合小麦T. timopheevii IL和染色体特异性KASP标记为小麦育种者提供了宝贵的资源，用于发现性状以对抗由于气候变化而影响小麦生产的生物和非生物胁迫因素。
Triticum timopheevii (2n = 28, AtAtGG) is a tetraploid wild relative species with great potential to increase the genetic diversity of hexaploid wheat Triticum aestivum (2n = 42, AABBDD) for various important agronomic traits. A breeding scheme that propagated advanced backcrossed populations of wheat-T. timopheevii introgression lines through further backcrossing and self-fertilisation resulted in the generation of 99 introgression lines (ILs) that carried 309 homozygous segments from the At and G subgenomes of T. timopheevii. These introgressions contained 89 and 74 unique segments from the At and G subgenomes, respectively. These overlapping segments covered 98.9% of the T. timopheevii genome that has now been introgressed into bread wheat cv. Paragon including the entirety of all T. timopheevii chromosomes via varying sized segments except for chromosomes 3At, 4G, and 6G. Homozygous ILs contained between one and eight of these introgressions with an average of three per introgression line. These homozygous introgressions were detected through the development of a set of 480 chromosome-specific Kompetitive allele specific PCR (KASP) markers that are well-distributed across the wheat genome. Of these, 149 were developed in this study based on single nucleotide polymorphisms (SNPs) discovered through whole genome sequencing of T. timopheevii. A majority of these KASP markers were also found to be T. timopheevii subgenome specific with 182 detecting At subgenome and 275 detecting G subgenome segments. These markers showed that 98% of the At segments had recombined with the A genome of wheat and 74% of the G genome segments had recombined with the B genome of wheat with the rest recombining with the D genome of wheat. These results were validated through multi-colour in situ hybridisation analysis. Together these homozygous wheat-T. timopheevii ILs and chromosome-specific KASP markers provide an invaluable resource to wheat breeders for trait discovery to combat biotic and abiotic stress factors affecting wheat production due to climate change.
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影响因子：
1.6
作者：
Hu, X. G.;Liu, J.;Zheng, Y. L.
通讯作者：
Zheng, Y. L.
影响因子：
4.5
作者：
BrownGuedira, GL;Gill, BS;Zwer, PK
通讯作者：
Zwer, PK
影响因子：
5.6
作者：
Grewal S;Guwela V;Newell C;Yang CY;Ashling S;Scholefield D;Hubbart-Edwards S;Burridge A;Stride A;King IP;King J
通讯作者：
King J
使用下一代 DNA 测序数据进行变异发现和基因分型的框架。
影响因子：
30.8
作者：
通讯作者：
--
通过全基因组渗入小麦的脑膜膜染色体表征。
DOI：
10.1007/s00122-017-3009-y
发表时间：
2018-03
期刊：
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
影响因子：
--
作者：
Grewal S;Yang C;Edwards SH;Scholefield D;Ashling S;Burridge AJ;King IP;King J
通讯作者：
King J