Characterisation of Thinopyrum bessarabicum chromosomes through genome-wide introgressions into wheat.
Characterisation of Thinopyrum bessarabicum chromosomes through genome-wide introgressions into wheat.
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通过全基因组渗入小麦的脑膜膜染色体表征。
DOI：
10.1007/s00122-017-3009-y
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发表时间：
2018-03
期刊：
影响因子：
--
通讯作者：
King J
中科院分区：
文献类型：
--
作者：
Grewal S;Yang C;Edwards SH;Scholefield D;Ashling S;Burridge AJ;King IP;King J
将百萨偃麦草全基因组基因组导入小麦，获得12个重组株系。细胞学和分子生物学技术允许在J基因组的所有7条染色体上绘制1150个SNP标记。 白萨偃麦草（Thinopyrumbessarabicum，2n = 2x = 14，JJ）具有耐盐、抗病等优良特性，是小麦遗传改良的重要变异来源。它在小麦改良中的实际应用可以通过全基因组渐渗的发展来促进，从而产生各种不同的小麦-Th。bessarabicum易位系在这项研究中，我们报告了12个这样的小麦-Th。通过两种不同的杂交策略，获得了10个白芥重组株系，并利用顺序单色和多色基因组原位杂交（sc-GISH和mc-GISH）、多色荧光原位杂交（mc-FISH）和单核苷酸多态性（SNP）DNA标记对它们进行了鉴定。我们还检测到13个线含有不同的Th。bessarabicum染色体畸变。对25个含Th的株系进行分子和细胞学分析。bessarabicum重组体和染色体畸变，我们能够物理地将1150个SNP标记定位到7个Th. Bessarabicum J染色体分为36个片段块。Th. bessarabicum和小麦基因组的同源性分析表明，两个物种之间的同线性在宏观水平上是高度保守的，并证实了Th. bessarabicum含有也存在于小麦A基因组中的4/5易位。这些小麦bessarabicum重组系和SNP标记为小麦改良提供了有用的遗传资源，后者作为检测来自含有J或密切相关基因组的其它偃麦草属物种（例如中间偃麦草（JrJrJvsJvsStSt）和长穗偃麦草（EeeEe））的基因渗入的工具具有更广泛的影响。本文的在线版本（10.1007/s 00122 -017-3009-y）包含补充材料，可供授权用户使用。
Genome-wide introgressions of Thinopyrum bessarabicum into wheat resulted in 12 recombinant lines. Cytological and molecular techniques allowed mapping of 1150 SNP markers across all seven chromosomes of the J genome. Thinopyrum bessarabicum (2n = 2x = 14, JJ) is an important source for new genetic variation for wheat improvement due to its salinity tolerance and disease resistance. Its practical utilisation in wheat improvement can be facilitated through development of genome-wide introgressions leading to a variety of different wheat–Th . bessarabicum translocation lines. In this study, we report the generation of 12 such wheat–Th . bessarabicum recombinant lines, through two different crossing strategies, which were characterized using sequential single colour and multi-colour genomic in situ hybridization (sc-GISH and mc-GISH), multi-colour fluorescent in situ hybridization (mc-FISH) and single nucleotide polymorphic (SNP) DNA markers. We also detected 13 lines containing different Th. bessarabicum chromosome aberrations through sc-GISH. Through a combination of molecular and cytological analysis of all the 25 lines containing Th. bessarabicum recombinants and chromosome aberrations we were able to physically map 1150 SNP markers onto seven Th. bessarabicum J chromosomes which were divided into 36 segmental blocks. Comparative analysis of the physical map of Th. bessarabicum and the wheat genome showed that synteny between the two species is highly conserved at the macro-level and confirmed that Th. bessarabicum contains the 4/5 translocation also present in the A genome of wheat. These wheat–Th . bessarabicum recombinant lines and SNP markers provide a useful genetic resource for wheat improvement with the latter having a wider impact as a tool for detection of introgressions from other Thinopyrum species containing the J or a closely-related genome such as Thinopyrum intermedium (JrJrJvsJvsStSt) and Thinopyrum elongatum (EeEe), respectively. The online version of this article (10.1007/s00122-017-3009-y) contains supplementary material, which is available to authorized users.
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影响因子：
3.8
作者：
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通讯作者：
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