High-resolution multipinhole single-photon-emission computed tomography in experimental and human arthritis.
High-resolution multipinhole single-photon-emission computed tomography in experimental and human arthritis.
复制标题
实验性关节炎和人类关节炎的高分辨率多针孔单光子发射计算机断层扫描。
作者：
Benedikt Ostendorf;Axel Scherer;A. Wirrwar,;J. Hoppin;C. Lackas;N. Schramm;M. Cohnen;Ulrich Mödder;W. Berg;Hans;Matthias Schneider;L. Joosten
客观化
使用新开发的高分辨率多针孔单光子发射计算机断层扫描(MPH-SPECT)技术，对实验性关节炎和关节炎患者的炎性关节炎病变进行成像。
方法
对6只白细胞介素1受体拮抗剂拮抗剂缺陷的前后爪关节炎小鼠和2只对照BALB/c小鼠进行MPH-SPECT扫描，并对关节炎进行大体评分。SPECT成像是用常规伽马相机进行的，升级后的金字塔铅准直器上贴着MPH孔。所有的图像都被重建，爪子的摄取以计数/重量和注射活动来量化。为了将成像技术转移到人类身上，我们检查了6名患者的临床优势手(3名确诊的类风湿性关节炎，1名早期类风湿性关节炎，1名骨性关节炎，1名健康对照)。
结果
MPH-SPECT图像是高分辨率的三维断层图像，可以对骨代谢增加进行准确的定位和定量观察。小鼠炎症关节的MPH-SPECT计数与死后的宏观评分和组织学关节分析相关。在人类中，MPH-SPECT图像描绘了示踪剂在手和手指关节的骨结构中积累的详细可视化，并能够成像在其他成像方式(如磁共振成像)中看起来正常的骨代谢增加。
结论
MPH-SPECT技术代表了一种新的诊断工具，用于检测小动物关节炎研究中的骨病理。与宏观评分相比，这种新的方法提供了更客观和更高精度的骨反应的量化测量，允许在体内可视化整个骨骼的炎症过程。这些结果表明，MPH-SPECT可作为监测实验性关节炎的一种有用的诊断工具，并具有进一步用于人类类风湿关节炎研究的潜力。
OBJECTIVE
To image inflammatory arthritic lesions in experimental arthritis and in patients with arthritis, using a newly developed high-resolution multipinhole single-photon-emission computed tomography (MPH-SPECT) technique.
METHODS
Six interleukin-1 receptor antagonist-deficient mice with arthritis of the front and back paws and 2 control BALB/c mice were imaged with MPH-SPECT and scored macroscopically for arthritis. SPECT imaging was performed with a conventional gamma camera upgraded with a pyramidal lead collimator affixed with MPH apertures. All images were reconstructed, and uptake in the paws was quantified in counts/weight and injected activity. To transfer the imaging technique to humans we examined the clinically dominant hand of 6 individuals (3 with established rheumatoid arthritis [RA], 1 with early RA, 1 with osteoarthritis, and 1 healthy control).
RESULTS
MPH-SPECT images were high-resolution 3-dimensional tomographic images, which allowed exact localization and quantifiable observation of increased bone metabolism. MPH-SPECT counts of inflamed joints in mice correlated with macroscopic scoring and histologic joint analysis postmortem. In humans, MPH-SPECT images depicted a detailed visualization of tracer accumulation in bony structures of hand and finger joints, and were also capable of imaging increased bone metabolism that had appeared normal with other imaging modalities, e.g., magnetic resonance imaging.
CONCLUSION
The MPH-SPECT technique represents a new diagnostic tool in the detection of bone pathology in small-animal arthritis research. Compared with macroscopic scoring, this new method provides a more objective and higher-precision quantifiable measurement of bone reaction, allowing visualization of inflammatory processes of the whole skeleton in vivo. These results suggest that MPH-SPECT may be useful as a diagnostic instrument for monitoring experimental arthritis, with further potential for use in human studies of RA.
影响因子：
--
作者：
Wunder, A;Tung, CH;Mahmood, U
通讯作者：
Mahmood, U
铟-111-白细胞、镓-67-多克隆 IgG 和其他放射性物质在急性局灶性炎症病变中的定位。
DOI：
--
发表时间：
1991
期刊：
Journal of nuclear medicine : official publication, Society of Nuclear Medicine
影响因子：
--
作者：
McAfee,JG;Gagne,G;Subramanian,G;Schneider,RF
通讯作者：
Schneider,RF
影响因子：
2.5
作者：
Dardzinski, BJ;Schmithorst, VJ;Hirsch, R
通讯作者：
Hirsch, R