A Novel Transgenic Mouse Line for Tracing MicroRNA-155-5p Activity In Vivo.
A Novel Transgenic Mouse Line for Tracing MicroRNA-155-5p Activity In Vivo.
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用于追踪 MicroRNA-155-5p 体内活性的新型转基因小鼠品系
DOI：
10.1371/journal.pone.0128198
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Zhou X
中科院分区：
文献类型：
--
作者：
Phiwpan K;Guo J;Zhang W;Hu T;Boruah BM;Zhang J;Zhou X
microRNA-155（miR-155）在先天免疫和获得性免疫的多种生理过程中发挥重要作用。miR-155表达水平在各种免疫应答期间动态变化。然而，目前用于在RNA水平检测miR-155的方法不能精确地反映实时活性。在此，我们通过将miR-155 - 5 p靶序列插入包含白喉毒素受体和TagBlue荧光蛋白的报告转基因的下游，产生了用于体内测定miR-155 - 5 p活性的转基因小鼠系（R26-DTR-155 T）。使用这种方法，R26-DTR-155 T小鼠能够测量特定细胞类型中miR-155- 5 p活性水平的变化。DTR表达水平与通过定量RT-PCR检测的内源性miR-155表达模式呈负相关。我们的数据证明了一种新的转基因小鼠系，其可用于通过在单细胞水平测量miR-155 - 5 p活性来追踪特定细胞类型中的miR-155 - 5 p活性。
MicroRNA-155 (miR-155) plays significant role in various physiological processes involving both innate and adaptive immunity. miR-155 expression level changes dynamically during various immune responses. However, current approaches for miR-155 detection at the RNA level do not precisely reflect the real-time activity. Herein, we generated a transgenic mouse line (R26-DTR-155T) for determination of miR-155-5p activity in vivo by inserting miR-155-5p target sequence downstream of a reporter transgene comprising Diphtheria Toxin Receptor and TagBlue fluorescence protein. Using this approach, R26-DTR-155T mice were able to measure variation in levels of miR-155-5p activity in specific cell types of interest. The DTR expression levels were inversely correlated with the endogenous miR-155 expression pattern as detected by quantitative RT-PCR. Our data demonstrate a novel transgenic mouse line which could be useful for tracing miR-155-5p activity in specific cell types through measurement of miR-155-5p activity at single cell level.
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影响因子：
32.4
作者：
O'Connell RM;Kahn D;Gibson WS;Round JL;Scholz RL;Chaudhuri AA;Kahn ME;Rao DS;Baltimore D
通讯作者：
Baltimore D
DOI：
10.1038/nri3494
发表时间：
2013-09
期刊：
Nature reviews. Immunology
影响因子：
--
作者：
通讯作者：
--
影响因子：
14.9
作者：
Ezzine S;Vassaux G;Pitard B;Barteau B;Malinge JM;Midoux P;Pichon C;Baril P
通讯作者：
Baril P
DOI：
10.1126/science.1139253
发表时间：
2007-04-27
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Rodriguez A;Vigorito E;Clare S;Warren MV;Couttet P;Soond DR;van Dongen S;Grocock RJ;Das PP;Miska EA;Vetrie D;Okkenhaug K;Enright AJ;Dougan G;Turner M;Bradley A
通讯作者：
Bradley A
DOI：
10.1016/j.molbrainres.2004.07.002
发表时间：
2004-11-04
期刊：
MOLECULAR BRAIN RESEARCH
影响因子：
--
作者：
Alexander, GM;Erwin, KL;Heiman-Patterson, TD
通讯作者：
Heiman-Patterson, TD