Integration of single-cell transcriptomes and chromatin landscapes reveals regulatory programs driving pharyngeal organ development.
Integration of single-cell transcriptomes and chromatin landscapes reveals regulatory programs driving pharyngeal organ development.
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DOI：
10.1038/s41467-022-28067-4
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发表时间：
2022-01-24
影响因子：
16.6
通讯作者：
Maehr R
中科院分区：
文献类型：
--
作者：
Magaletta ME;Lobo M;Kernfeld EM;Aliee H;Huey JD;Parsons TJ;Theis FJ;Maehr R
Maldevelopment of the pharyngeal endoderm, an embryonic tissue critical for patterning of the pharyngeal region and ensuing organogenesis, ultimately contributes to several classes of human developmental syndromes and disorders. Such syndromes are characterized by a spectrum of phenotypes that currently cannot be fully explained by known mutations or genetic variants due to gaps in characterization of critical drivers of normal and dysfunctional development. Despite the disease-relevance of pharyngeal endoderm, we still lack a comprehensive and integrative view of the molecular basis and gene regulatory networks driving pharyngeal endoderm development. To close this gap, we apply transcriptomic and chromatin accessibility single-cell sequencing technologies to generate a multi-omic developmental resource spanning pharyngeal endoderm patterning to the emergence of organ-specific epithelia in the developing mouse embryo. We identify cell-type specific gene regulation, distill GRN models that define developing organ domains, and characterize the role of an immunodeficiency-associated forkhead box transcription factor. The molecular basis and gene regulatory networks driving pharyngeal endoderm development remain poorly understood. Here the authors report single cell transcriptomic and chromatin landscapes to delineate regulatory programs driving this process and to define the immunodeficiency-associated developmental defects resulting from Foxn1 dysfunction.
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影响因子：
16.6
作者：
Bleu M;Mermet-Meillon F;Apfel V;Barys L;Holzer L;Bachmann Salvy M;Lopes R;Amorim Monteiro Barbosa I;Delmas C;Hinniger A;Chau S;Kaufmann M;Haenni S;Berneiser K;Wahle M;Moravec I;Vissières A;Poetsch T;Ahrné E;Carte N;Voshol J;Bechter E;Hamon J;Meyerhofer M;Erdmann D;Fischer M;Stachyra T;Freuler F;Gutmann S;Fernández C;Schmelzle T;Naumann U;Roma G;Lawrenson K;Nieto-Oberhuber C;Cobos-Correa A;Ferretti S;Schübeler D;Galli GG
通讯作者：
Galli GG
影响因子：
2.6
作者：
Becker, MB;Zülch, A;Gruss, P
通讯作者：
Gruss, P
影响因子：
2.7
作者：
Desai, TJ;Malpel, S;Cardoso, WV
通讯作者：
Cardoso, WV
影响因子：
64.5
作者：
Calderon, Lesly;Boehm, Thomas
通讯作者：
Boehm, Thomas
DOI：
10.1073/pnas.93.12.5742
发表时间：
1996-06-11
影响因子：
11.1
作者：
Blackburn, CC;Augustine, CL;Morahan, G
通讯作者：
Morahan, G