Exploring traditional aus-type rice for metabolites conferring drought tolerance.
Exploring traditional aus-type rice for metabolites conferring drought tolerance.
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探索传统的澳大利亚大米，以赋予耐旱性的代谢产物。
DOI：
10.1186/s12284-017-0189-7
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发表时间：
2018-01-25
期刊：
影响因子：
--
通讯作者：
Heuer S
中科院分区：
文献类型：
--
作者：
Casartelli A;Riewe D;Hubberten HM;Altmann T;Hoefgen R;Heuer S
水稻的传统品种和地方品种属于austype组（Oryza sativa L.）已知其对环境胁迫如干旱和高温具有高度耐受性，因此被认为是作物改良的宝贵遗传资源。以2个aus型（Dular，N22）和2个耐旱灌溉品种（IR 64，IR 74）为材料，进行了非靶向代谢组学分析，以鉴定与耐旱性相关的干旱响应代谢物。Dular和N22与灌溉品种相比具有上级耐旱性，通过在干旱处理中施加严重干旱胁迫后生长至成熟的植物的表型来证实。Dular和N22并没有表现出显着减少的谷物产量相比，浇水良好的对照植物，而不耐品种表现出显着减少，总小穗数和谷物产量。在干旱处理结束时的分蘖期，用植物的茎和根样品进行代谢组学分析。数据显示，总体上较高的积累的N-丰富的代谢产物（氨基酸和核苷酸相关的代谢产物尿囊素和尿苷）的耐受品种的芽。在根中，aus型品种的特征在于代表糖酵解和TCA循环的代谢物的更高的减少，例如苹果酸、甘油酸和甘油酸-3-磷酸。另一方面，低聚糖棉子糖在敏感基因型的芽和根中均显示出较高的倍数增加。数据进一步表明，对于某些干旱反应代谢物，对比水稻品种之间的差异在充分浇水的对照条件下已经很明显。在aus类型品种中鉴定的耐旱性相关代谢物提供了一组有价值的保护性化合物，并为评估开发耐旱水稻和其他作物的潜在途径的遗传多样性提供了一个切入点。本文的在线版本（10.1186/s12284-017-0189-7）包含补充材料，可供授权用户使用。
Traditional varieties and landraces belonging to the aus-type group of rice (Oryza sativa L.) are known to be highly tolerant to environmental stresses, such as drought and heat, and are therefore recognized as a valuable genetic resource for crop improvement. Using two aus-type (Dular, N22) and two drought intolerant irrigated varieties (IR64, IR74) an untargeted metabolomics analysis was conducted to identify drought-responsive metabolites associated with tolerance. The superior drought tolerance of Dular and N22 compared with the irrigated varieties was confirmed by phenotyping plants grown to maturity after imposing severe drought stress in a dry-down treatment. Dular and N22 did not show a significant reduction in grain yield compared to well-watered control plants, whereas the intolerant varieties showed a significant reduction in both, total spikelet number and grain yield. The metabolomics analysis was conducted with shoot and root samples of plants at the tillering stage at the end of the dry-down treatment. The data revealed an overall higher accumulation of N-rich metabolites (amino acids and nucleotide-related metabolites allantoin and uridine) in shoots of the tolerant varieties. In roots, the aus-type varieties were characterised by a higher reduction of metabolites representative of glycolysis and the TCA cycle, such as malate, glyceric acid and glyceric acid-3-phosphate. On the other hand, the oligosaccharide raffinose showed a higher fold increase in both, shoots and roots of the sensitive genotypes. The data further showed that, for certain drought-responsive metabolites, differences between the contrasting rice varieties were already evident under well-watered control conditions. The drought tolerance-related metabolites identified in the aus-type varieties provide a valuable set of protective compounds and an entry point for assessing genetic diversity in the underlying pathways for developing drought tolerant rice and other crops. The online version of this article (10.1186/s12284-017-0189-7) contains supplementary material, which is available to authorized users.
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DOI：
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发表时间：
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期刊：
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影响因子：
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DOI：
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发表时间：
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期刊：
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影响因子：
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