The rat glomerular filtration barrier does not show negative charge selectivity.
The rat glomerular filtration barrier does not show negative charge selectivity.
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大鼠肾小球滤过屏障不显示负电荷选择性。
DOI：
10.1038/sj.mn.7800150
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Carbajal,JoséM
中科院分区：
文献类型：
--
作者：
SchaefferJr,RichardC;Gratrix,MaxL;Mucha,DavidR;Carbajal,JoséM
目的：通过筛分曲线表征大小、形状和负电荷对大分子穿过肾小球毛细血管壁转运的影响（分数清除率对溶质分子半径）的荧光多分散多糖示踪剂。用中性荧光[等电点（pI）= 7.3 ± 0.2]和带负电的荧光测定肾小球清除率（FC）（pI = 3.5 ± 0.4）葡聚糖（DEX）与带负电荷（pI = 4.8 ± 0.3）羟乙基淀粉（HES）和（pI = 4.6 ± 0.1）牛血清白蛋白（BSA）在Sprague-道利和Fischer 344/Brown Norway大鼠中的比较。通过使用14 C-菊粉的尿清除率测量FC（n= 53）以确定肾小球滤过率。通过使用冷却的（−25 °C）CCD照相机在显微镜下测量内皮细胞和肾近端小管上皮细胞（LLC-PK 1）在体外对每种荧光探针的相对摄取。结果：随机卷曲的中性和带负电荷的DEX探针的筛分曲线是相同的。 这些FC值比HES高6倍，比类似大小的荧光BSA高200倍。多糖探针未显示出与血清蛋白的显著结合。LLC-PK 1细胞对BSA的摄取是中性或带负电荷的大分子的20 - 100倍。结论：这些发现表明，大鼠肾小球滤过屏障限制了多糖大分子的转运，其大小和构型是其功能，但不是负电荷。
Objective: To characterize the effects of size, shape, and negative charge on the transport of macromolecules across the glomerular capillary wall by using the sieving curves (fractional clearance vs. solute molecular radii) of fluorescent polydispersed polysaccharide tracers.Methods: Glomerular fractional clearances (FC) were measured with fluorescent neutral [isoelectric point (pI) = 7.3 ± 0.2] and negatively charged (pI = 3.5 ± 0.4) dextrans (DEX) in comparison with negatively charged (pI = 4.8 ± 0.3) hydroxy ethyl starch (HES) and (pI = 4.6 ± 0.1) bovine serum albumin (BSA) in Sprague‐Dawley and Fischer 344/Brown Norway rats. FCs (n= 53) were measured by using the urinary clearance of14C‐inulin to determine the glomerular filtration rate. The relative uptake of each fluorescent probe by endothelial and renal proximal tubule epithelial (LLC‐PK1) cells,in vitro, was measured microscopically by using a cooled (−25 °C) CCD camera.Results: The sieving curves for randomly coiled neutral and negatively charged DEX probes were identical. These FC values were 6‐fold greater than those for HES and 200‐fold above similarly sized fluorescent BSA. The polysaccharide probes did not show significant binding to serum proteins. The uptake of BSA by LLC‐PK1cells was 20‐ to 100‐fold greater than that for neutral or negatively charged macromolecules.Conclusions: These findings indicate that the rat glomerular filtration barrier restricts the transport of polysaccharide macromolecules as a function of their size and configuration but not negative charge.
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