Distribution and Seasonal Variations in Levels of Three Native GnRHs in the Brain and Pituitary of Perciform Fish
Distribution and Seasonal Variations in Levels of Three Native GnRHs in the Brain and Pituitary of Perciform Fish
复制标题
鲈形鱼大脑和垂体中三种天然GnRHs的分布和水平的季节变化
DOI：
10.1046/j.1365-2826.1999.00304.x
复制
发表时间：
1999
影响因子：
3.2
通讯作者：
Kagawa
中科院分区：
文献类型：
--
作者：
Senthilkumaran;Okuzawa;Gen;Ookura;Kagawa
新开发了两种类型促性腺激素释放激素（GnRH）的特异性和灵敏度放射免疫测定法（RIA），即海鸟（sb）GnRH和鸡（c）GnRH-II。我们采用这两种RIA和先前报道的鲑鱼GnRH RIA研究了这三种GnRH在四种鲈形目鱼类脑和垂体中的存在和区域分布（红鲷，Pagrus major;黑鲷，Schlegeli;条纹刀颌鲷，Oplegnathus fasciatus;和尼罗罗非鱼，Oreochromis niloticus），以及澄清季节性变化的水平，这些促性腺激素释放激素在大脑和脑垂体的红海鸟。所有这三种GnRHs都存在于所有鱼类的大脑中，这三种GnRHs在大脑中的区域分布相当相似。端脑和下丘脑sbGnRH含量丰富。cGnRH-II从大脑的中后部集中，分布于整个大脑。sGnRH集中在嗅球中，并分布在整个大脑中，cGnRH-II也是如此。垂体中GnRH的主要形式是sbGnRH，其水平比sGnRH高500至2400倍，而cGnRH-II在所有四种物种中均检测不到。在雌性红鲷的脑和垂体中，脑和垂体sbGnRH水平从10月（未成熟期）开始增加，并在4月（产卵期）达到高峰，反映了性腺指数和卵黄发生的增加。然而，sbGnRH水平仍然很高，只有在脑垂体完全退化的鱼在6月。脑中sGnRH和cGnRH-II的水平在回归期较高，在产卵期保持较低。从这些和以前的结果，它似乎sbGnRH是生理上最重要的形式的GnRH在繁殖中的红海鸟，并可能在其他鲈形目也。
Specific and sensitive radioimmunoassays (RIAs) were newly developed for two types of gonadotropin‐releasing hormone (GnRH), namely, seabream (sb) GnRH and chicken (c) GnRH‐II. We employed these two RIAs together with a previously reported RIA for salmon (s) GnRH to study the presence and regional distribution of these three GnRHs in the brains and pituitaries of four perciform fishes (red seabream, Pagrus major; black seabream, Acanthopagrus schlegeli; striped knifejaw, Oplegnathus fasciatus; and Nile tilapia, Oreochromis niloticus), as well as clarify seasonal changes in levels of these GnRHs in the brain and pituitary of red seabream. All three GnRHs were found in brains of all fishes examined, with regional distributions in the brains of the three GnRHs being rather similar. sbGnRH was abundant in telencephalon and hypothalamus. cGnRH‐II was concentrated from the middle to posterior part of the brain and distributed throughout the brain. sGnRH was concentrated in the olfactory bulb and distributed all over the brain, as was cGnRH‐II. The dominant form of GnRH in the pituitary was sbGnRH, with levels 500‐ to 2400‐fold higher than those of sGnRH, while cGnRH‐II was undetectable in all four species. In the brain and pituitary of female red seabream, levels of both brain and pituitary sbGnRH increased from October (immature phase) and reached a peak in April (spawning phase), reflecting the increase in gonadosomatic index and vitellogenesis. However, levels of sbGnRH remained high only in the pituitary of completely regressed fish in June. Levels of both sGnRH and cGnRH‐II in the brain were higher in the regressed phase and remained lower during the spawning phase. From these and previous results, it appears that sbGnRH is physiologically the most important form of GnRH in reproduction in red seabream and, probably, in other perciforms also.
DOI：
10.1073/pnas.91.25.12081
发表时间：
1994-12
影响因子：
11.1
作者：
J. Powell;Yonathan Zohar;A. Elizur;Minkyu Park;Wolfgang H. Fischer;A. Craig;J. Rivier;David A. Lovejoy;N. Sherwood
通讯作者：
J. Powell;Yonathan Zohar;A. Elizur;Minkyu Park;Wolfgang H. Fischer;A. Craig;J. Rivier;David A. Lovejoy;N. Sherwood
一个生物体中的三个促性腺激素释放激素基因表明了一种古老肽的新作用。
DOI：
10.1073/pnas.92.18.8363
发表时间：
1995
影响因子：
11.1
作者：
White,SA;Kasten,TL;Bond,CT;Adelman,JP;Fernald,RD
通讯作者：
Fernald,RD