Evidence for coupling between steady-state and dynamic extracellular dopamine concentrations in the rat striatum.
Evidence for coupling between steady-state and dynamic extracellular dopamine concentrations in the rat striatum.
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DOI：
10.1111/j.1471-4159.2010.06740.x
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发表时间：
2010-07
影响因子：
4.7
通讯作者：
Michael AC
中科院分区：
文献类型：
--
作者：
Wang Y;Moquin KF;Michael AC
我们实验室以前的一项研究表明，大鼠纹状体内多巴胺能末梢处于不同的动态状态，至少部分取决于末梢受自抑制的程度。本研究旨在检验基础紧张性细胞外多巴胺浓度的异质性导致自抑制程度不同的假设。我们探测基础细胞外多巴胺浓度使用先前证明的策略，利用纹状体内微量输注犬尿氨酸，一种物质，根据伏安测量降低细胞外多巴胺从其基础浓度。然而，在纹状体中，我们发现对犬尿氨酸输注的反应本身是异质的，允许将纹状体内的位点分别广泛分类为犬尿氨酸不敏感和犬尿氨酸敏感。这些新发现的犬尿酸不敏感和敏感的网站产生实质上和显着不同的诱发DA释放，通过伏安法测量在电刺激内侧前脑束。我们的研究结果证实了这一假设，即在当地的基础浓度的DA的异质性是负责纹状体内的自抑制的可变程度，并支持的结论，在这个大脑区域的细胞外DA的稳态和动态组件耦合。
A previous study from our laboratory demonstrated the presence within the rat striatum of dopaminergic terminals in different dynamical states, determined at least in part by the extent to which terminals are subject to autoinhibition. The present study is designed to test the hypothesis that heterogeneity in the basal tonic extracellular dopamine concentration contributes to the variable extent of autoinhibition. We probed basal extracellular dopamine concentrations using a previously demonstrated strategy that utilizes intrastriatal microinfusion of kynurenate, a substance that according to voltammetric measurements decreases extracellular dopamine from its basal concentration. In the striatum, however, we find that the response to kynurenate infusion is itself heterogeneous, allowing a broad classification of sites within the striatum as kynurenate-insensitive and kynurenate-sensitive, respectively. These newly identified kynurenate-insensitive and sensitive sites yield substantially and significantly different evoked DA release as measured by voltammetry during electrical stimulation of the medial forebrain bundle. Our findings confirm the hypothesis that heterogeneity in the local basal concentration of DA is responsible for the variable extent of autoinhibition within the striatum and support the conclusion the steady state and dynamical components of extracellular DA in this brain region are coupled.
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DOI：
10.1073/pnas.1834314100
发表时间：
2003-09-30
影响因子：
11.1
作者：
Avshalumov, MV;Rice, ME
通讯作者：
Rice, ME
影响因子：
4.7
作者：
Kita, Justin M.;Parker, Lauren E.;Wightman, R. Mark
通讯作者：
Wightman, R. Mark
影响因子：
56.9
作者：
EWING, AG;BIGELOW, JC;WIGHTMAN, RM
通讯作者：
WIGHTMAN, RM
影响因子：
5.3
作者：
Benoit-Marand, M;Borrelli, E;Gonon, F
通讯作者：
Gonon, F
影响因子：
3.3
作者：
Kulagina, NV;Zigmond, MJ;Michael, AC
通讯作者：
Michael, AC