Semicontinuous flow electroporation chip for high-throughput transfection on mammalian cells.
Semicontinuous flow electroporation chip for high-throughput transfection on mammalian cells.
复制标题
DOI：
10.1021/ac9002672
复制
发表时间：
2009-06-01
影响因子：
7.4
通讯作者：
Lee, L. James
中科院分区：
文献类型：
--
作者：
Wang, Shengnian;Zhang, Xulang;Wang, Weixiong;Lee, L. James
We have recently developed a semi-continuous flow electroporation (SFE) device for in vitro DNA delivery. Cells mixed with plasmid DNA continuously flowed through a serpentine channel, the side walls of which also serving as electrodes. Using pWizGFP plasmid and K562 cells as a model system, SFE showed better transgene expression (10~15%) compared to a commercial electroporation system. Quantitative results via MTS assay also revealed a 50% or higher cell viability. Similar observations were also found with pWizGFP transfection to mouse embryonic stem cells. Such improvements were attributed to less gas formation and joule heating in SFE.
登录
查看更多内容
影响因子：
6.1
作者：
Erickson, D;Sinton, D;Li, DQ
通讯作者：
Li, DQ
影响因子：
6.1
作者：
Khine, M;Lau, A;Lee, LP
通讯作者：
Lee, LP
影响因子：
4.6
作者：
Huang, Y;Rubinsky, B
通讯作者：
Rubinsky, B
影响因子：
6.1
作者：
Lee, Won Gu;Bang, Hyunwoo;Han, Dong-Chul
通讯作者：
Han, Dong-Chul
影响因子：
8.4
作者：
Lin, YC;Jen, CM;Lin, XZ
通讯作者：
Lin, XZ