Wolbachia wPip Blocks Zika Virus Transovarial Transmission in Aedes albopictus.
Wolbachia wPip Blocks Zika Virus Transovarial Transmission in Aedes albopictus.
复制标题
DOI：
10.1128/spectrum.02633-21
复制
发表时间：
2022-10-26
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
--
作者：
沃尔巴克氏体正在被开发为一种生物工具，用于抑制蚊子种群和/或干扰其传播的病毒。人工感染沃尔巴克氏体的成年雄性已被释放，在多项现场试验中成功抑制了种群数量。用于抑制计划的人工感染沃尔巴克氏体的蚊子的主要特征是在水平和垂直传播方面比本地感染/未感染的蚊子的媒介能力较低。我们之前的研究表明，感染三种沃尔巴克氏体菌株的白纹伊蚊HC系几乎完全阻断登革热病毒（DENV）和寨卡病毒（ZIKV）的水平和垂直传播。然而，沃尔巴克氏体抑制病毒经卵巢传播的程度尚不清楚，因为尚未进行研究来确定沃尔巴克氏体是否保护卵巢细胞免受病毒感染。在这里，我们使用了伊蚊的卵巢细胞。研究人员对白纹伊蚊 GUA（一种野生型蚊子系，双重感染两种本地沃尔巴克氏体菌株 wAlbA 和 wAlbB）、HC 和 GT 系（经四环素治愈、未感染沃尔巴克氏体的蚊子）表现出关键特征，并对它们进行比较，以更好地了解沃尔巴克氏体如何抑制 ZIKV 经卵巢传播。我们的结果表明，在第一和第二个促性腺营养周期中，成年 GT 后代的感染率显着高于 GUA 后代。相比之下，成年 GT 和 GUA 后代的感染率在第三个促性腺营养周期没有显着差异。所有来自三个促性腺营养周期的成年 HC 后代的 ZIKV 感染均为阴性。蚊子卵巢中沃尔巴克氏体密度与 ZIKV 载量之间存在很强的负线性相关性。尽管沃尔巴克氏体和 ZIKV 的卵巢中没有明显的共存区域，但宿主免疫反应可能在沃尔巴克氏体阻止 ZIKV 在伊蚊卵巢中扩增和维持中发挥作用。白纹伊蚊。这些结果将有助于了解沃尔巴克氏体-寨卡病毒在蚊子中的相互作用。重要性 在多个国家大面积应用沃尔巴克氏体来抑制蚊子种群及其传播的病毒已取得成功。然而，大规模释放感染沃尔巴克氏体的雄性蚊子存在意外释放具有生育能力的雌性蚊子的潜在风险。在这项研究中，我们使用了伊蚊的卵巢细胞。白纹伊蚊 GUA、HC 和 GT 品系表现出关键性状，并对它们进行比较，以更好地了解沃尔巴克氏体如何抑制 ZIKV 经卵巢传播。我们的结果显示，HC 雌性蚊子中的 ZIKV 传播几乎被完全阻断。本地感染的 GUA 蚊子中的沃尔巴克氏体对 ZIKV 产生负面影响，并且这种干扰的负载量略低于 HC 蚊子中的负载量。总体而言，我们的工作有助于展示沃尔巴克氏体如何阻止 ZIKV 在伊蚊卵巢中的扩张和维持。白纹伊蚊和有助于了解沃尔巴克氏体-寨卡病毒在蚊子中的相互作用。
Wolbachia is being developed as a biological tool to suppress mosquito populations and/or interfere with their transmitted viruses. Adult males with an artificial Wolbachia infection have been released, successfully yielding population suppression in multiple field trials. The main characteristic of the artificial Wolbachia-infected mosquitoes used in the suppression program is the lower vector competence than that in native infected/uninfected mosquitoes in horizontal and vertical transmission. Our previous studies have demonstrated that the Aedes albopictus HC line infected with a trio of Wolbachia strains exhibited almost complete blockade of dengue virus (DENV) and Zika virus (ZIKV) in horizontal and vertical transmission. However, the extent to which Wolbachia inhibits virus transovarial transmission is unknown since no studies have been performed to determine whether Wolbachia protects ovarian cells against viral infection. Here, we employed ovarian cells of the Ae. albopictus GUA (a wild-type mosquito line superinfected with two native Wolbachia strains, wAlbA and wAlbB), HC, and GT lines (tetracycline-cured, Wolbachia-uninfected mosquitoes), which exhibit key traits, and compared them to better understand how Wolbachia inhibits ZIKV transovarial transmission. Our results showed that the infection rate of adult GT progeny was significantly higher than that of GUA progeny during the first and second gonotrophic cycles. In contrast, the infection rates of adult GT and GUA progeny were not significantly different during the third gonotrophic cycle. All examined adult HC progeny from three gonotrophic cycles were negative for ZIKV infection. A strong negative linear correlation existed between Wolbachia density and ZIKV load in the ovaries of mosquitoes. Although there is no obvious coexistence area in the ovaries for Wolbachia and ZIKV, host immune responses may play a role in Wolbachia blocking ZIKV expansion and maintenance in the ovaries of Ae. albopictus. These results will aid in understanding Wolbachia-ZIKV interactions in mosquitoes. IMPORTANCE Area-wide application of Wolbachia to suppress mosquito populations and their transmitted viruses has achieved success in multiple countries. However, the mass release of Wolbachia-infected male mosquitoes involves a potential risk of accidentally releasing fertile females. In this study, we employed ovarian cells of the Ae. albopictus GUA, HC, and GT lines, which exhibit key traits, and compared them to better understand how Wolbachia inhibits ZIKV transovarial transmission. Our results showed an almost complete blockade of ZIKV transmission in HC female mosquitoes. Wolbachia in natively infected GUA mosquitoes negative affected ZIKV, and this interference was shown by slightly lower loads than those in HC mosquitoes. Overall, our work helps show how Wolbachia blocks ZIKV expansion and maintenance in the ovaries of Ae. albopictus and aids in understanding Wolbachia-ZIKV interactions in mosquitoes.
影响因子：
13.2
作者：
Yin, Yingxian;Xu, Yi;Su, Ling;Zhu, Xun;Chen, Minxia;Zhu, Weijin;Xia, Huimin;Huang, Xi;Gong, Sitang
通讯作者：
Gong, Sitang