Silencing of S100A4, a metastasis-associated protein, in endothelial cells inhibits tumor angiogenesis and growth.
Silencing of S100A4, a metastasis-associated protein, in endothelial cells inhibits tumor angiogenesis and growth.
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DOI：
10.1007/s10456-013-9372-7
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发表时间：
2014-01
期刊：
影响因子：
9.8
通讯作者：
Endo, Hideya
中科院分区：
文献类型：
--
作者：
Ochiya, Takahiro;Takenaga, Keizo;Endo, Hideya
内皮细胞表达S100 A4，一种转移相关蛋白，但其在血管生成中的作用仍有待阐明。在这里，我们表明，敲低小鼠内皮细胞MSS 31细胞中的小鼠特异性小干扰RNA（mS 100 A4 siRNA）显着抑制毛细血管样管形成在体外，在治疗后的早期阶段，沿着下调和上调的一些促血管生成和抗血管生成基因的表达，分别。特别值得注意的是，在人前列腺癌异种移植物中肿瘤内施用mS 100 A4 siRNA显著减少了肿瘤血管分布并导致肿瘤生长的抑制。这些发现表明，S100 A4在内皮细胞中参与管形成，并表明其作为抑制肿瘤血管生成的分子靶点的潜力，这保证了进一步开发基于内皮S100 A4的癌症治疗策略。本文的在线版本（doi：10.1007/s10456-013-9372-7）包含补充材料，可供授权用户使用。
Endothelial cells express S100A4, a metastasis-associated protein, but its role in angiogenesis remains to be elucidated. Here we show that knockdown of S100A4 in mouse endothelial MSS31 cells by murine specific small interference RNA (mS100A4 siRNA) markedly suppressed capillary-like tube formation in vitro, in early stage after the treatment, along with down- and up-regulation of some of the pro-angiogenic and anti-angiogenic gene expression, respectively. Of particular note is that intra-tumor administration of the mS100A4 siRNA in a human prostate cancer xenograft significantly reduced tumor vascularity and resulted in the inhibition of tumor growth. These findings show that S100A4 in endothelial cells is involved in tube formation, and suggest its potential as a molecular target for inhibiting tumor angiogenesis, which warrants further development of endothelial S100A4-based strategies for cancer treatment. The online version of this article (doi:10.1007/s10456-013-9372-7) contains supplementary material, which is available to authorized users.
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DOI：
10.1038/newbio242148a0
发表时间：
1973-01-01
期刊：
NATURE-NEW BIOLOGY
影响因子：
--
作者：
FIDLER, IJ
通讯作者：
FIDLER, IJ
影响因子：
4.3
作者：
Lee, Sangjin;Nguyen, Tri M. Bui;Kovalenko, Dmitry;Adhikari, Neeta;Grindle, Suzanne;Polster, Sean P.;Friesel, Robert;Ramakrishnan, Sundaram;Hall, Jennifer L.
通讯作者：
Hall, Jennifer L.
影响因子：
3.7
作者：
Jurisic G;Iolyeva M;Proulx ST;Halin C;Detmar M
通讯作者：
Detmar M
影响因子：
10.8
作者：
Mu, H;Ohashi, R;Chen, CY
通讯作者：
Chen, CY
影响因子：
11.2
作者：
Grum-Schwensen, B;Klingelhofer, J;Ambartsumian, N
通讯作者：
Ambartsumian, N