Sequence elements critical for efficient RNA editing of a tobacco chloroplast transcript in vivo and in vitro.
Sequence elements critical for efficient RNA editing of a tobacco chloroplast transcript in vivo and in vitro.
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DOI：
10.1093/nar/gkl490
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发表时间：
2006
影响因子：
14.9
通讯作者：
Hanson MR
中科院分区：
文献类型：
--
作者：
Hayes ML;Reed ML;Hegeman CE;Hanson MR
In tobacco chloroplast transcripts 34 nt are efficiently edited to U. No common consensus region is present around all editing sites; however, sites can be grouped in clusters that share short common sequences. Transgene transcripts carrying either the wild-type −31/+22 or −31/+60 sequence near NTrpoB C473, an editing site within tobacco rpoB transcripts, or three different mutated sequences, were all highly edited in vivo. Endogenous transcripts of rpoB, psbL and rps14, all of which contain common sequences S1, S2 and S3 5′ to NTrpoB C473, NTpsbL C2 and NTrps14 C80, were less edited in transgenic plants that over-express transcripts from NTrpoB C473 transgenes. Extent of reduction of endogenous editing differed between transgenic lines expressing mutated −31/+22 regions, depending on the abundance of the transgene transcripts. The −20/−5 sequence contains critical 5′ sequence elements. Synthetic RNA templates with alterations within this 5′ region were less efficiently edited in vitro than wild-type templates, by either tobacco or maize chloroplast extracts. The tobacco chloroplast extract supports both RNA editing and processing of 3′ transcript termini. We conclude that within the −20/−5 region, sequences common to editing sites in the transcripts of rpoB, psbL and rps14 are critical for efficient NTrpoB C473 editing.
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DOI：
10.1073/pnas.0307163101
发表时间：
2004-01-06
影响因子：
11.1
作者：
Miyamoto, T;Obokata, J;Sugiura, M
通讯作者：
Sugiura, M
影响因子：
4.5
作者：
Neuwirt, J;Takenaka, M;Brennicke, A
通讯作者：
Brennicke, A
影响因子：
3.5
作者：
Reed, ML;Lyi, SM;Hanson, MR
通讯作者：
Hanson, MR
影响因子：
14.9
作者：
Handa, H
通讯作者：
Handa, H
影响因子：
5.3
作者：
Chateigner-Boutin, AL;Hanson, MR
通讯作者：
Hanson, MR