A smartphone-based particle diffusometry platform for sub-attomolar detection of Vibrio cholerae in environmental water.
A smartphone-based particle diffusometry platform for sub-attomolar detection of Vibrio cholerae in environmental water.
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DOI：
10.1016/j.bios.2020.112497
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发表时间：
2020-11-01
影响因子：
12.6
通讯作者：
Linnes JC
中科院分区：
文献类型：
--
作者：
Moehling TJ;Lee DH;Henderson ME;McDonald MK;Tsang PH;Kaakeh S;Kim ES;Wereley ST;Kinzer-Ursem TL;Clayton KN;Linnes JC
全世界每年有340万人死于水传播疾病。开发一个快速和便携式平台，用于检测和监测水传播病原体，将大大有助于减少传染病的发病率和传播。通过将光学方法和智能手机技术与分子检测相结合，可以很容易地实现检测极低浓度的水性病原体所需的灵敏度。在这里，我们实现了基于智能手机的颗粒扩散（PD）检测环介导等温扩增（LAMP）针对水生病原体霍乱弧菌（V.霍乱弧菌）。通过测量在智能手机上以68倍放大率成像的400 nm链霉亲和素包被的荧光纳米颗粒的扩散，我们可以在短短35分钟内检测到每个反应少至6个霍乱弧菌细胞（0.66 aM）。在132个池塘水样本的双盲研究中，我们建立了基于智能手机的PD平台检测霍乱弧菌的灵敏度为91.8%，特异性为95.2%，准确性为94.3%。总之，这些结果证明了这种基于智能手机的PD平台在使用时快速灵敏地检测霍乱弧菌的实用性。
Each year, 3.4 million people die from waterborne diseases worldwide. Development of a rapid and portable platform for detecting and monitoring waterborne pathogens would significantly aid in reducing the incidence and spread of infectious diseases. By combining optical methods and smartphone technology with molecular assays, the sensitivity required to detect exceedingly low concentrations of waterborne pathogens can readily be achieved. Here, we implement smartphone-based particle diffusometry (PD) detection of loop-mediated isothermal amplification (LAMP) targeting the waterborne pathogen Vibrio cholerae (V. cholerae). By measuring the diffusion of 400 nm streptavidin-coated fluorescent nanoparticles imaged at 68X magnification on a smartphone, we can detect as few as 6 V. cholerae cells per reaction (0.66 aM) in just 35 minutes. In a double-blinded study with 132 pond water samples, we establish a 91.8% sensitivity, 95.2% specificity, and 94.3% accuracy of the smartphone-based PD platform for detection of V. cholerae. Together, these results demonstrate the utility of this smartphone-based PD platform for rapid and sensitive detection of V. cholerae at the point of use.
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影响因子：
1.9
作者：
ADRIAN, RJ;YAO, CS
通讯作者：
YAO, CS
影响因子：
3.7
作者：
Nliwasa M;MacPherson P;Chisala P;Kamdolozi M;Khundi M;Kaswaswa K;Mwapasa M;Msefula C;Sohn H;Flach C;Corbett EL
通讯作者：
Corbett EL
影响因子：
4.6
作者：
Jiang L;Mancuso M;Lu Z;Akar G;Cesarman E;Erickson D
通讯作者：
Erickson D
影响因子：
2.4
作者：
Einstein, A
通讯作者：
Einstein, A
影响因子：
7.4
作者：
Clayton, Katherine N.;Berglund, Gregory D.;Wereley, Steven T.
通讯作者：
Wereley, Steven T.