Quantitative proteomic analysis of hepatocyte-secreted extracellular vesicles reveals candidate markers for liver toxicity.
Quantitative proteomic analysis of hepatocyte-secreted extracellular vesicles reveals candidate markers for liver toxicity.
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DOI：
10.1016/j.jprot.2014.04.008
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发表时间：
2014-05-30
影响因子：
3.3
通讯作者：
Falcón-Pérez JM
中科院分区：
文献类型：
--
作者：
Rodríguez-Suárez E;Gonzalez E;Hughes C;Conde-Vancells J;Rudella A;Royo F;Palomo L;Elortza F;Lu SC;Mato JM;Vissers JP;Falcón-Pérez JM
细胞外囊泡作为不同生物过程和疾病的生物标志物的可能来源引起了极大的兴趣。虽然这些囊泡的生物学功能尚未完全了解，但很明显，它们参与去除不必要的细胞物质，并作为细胞之间各种大分子和信号的载体。在这份报告中，我们分析了原代肝细胞分泌的细胞外囊泡的蛋白质组。我们使用一维和二维液相色谱结合数据独立的质谱。采用无标记的定量蛋白质组学，我们检测到囊泡蛋白表达水平的显着变化，在这个体外模型暴露后，众所周知的肝毒素（半乳糖胺和大肠杆菌衍生的脂多糖）。结果使我们能够确定肝损伤的候选标志物。我们在体内验证了这些标记物的数量，为开发新的方法来评估药物毒性提供了基础。这份报告强烈支持蛋白质组学在研究细胞外囊泡释放良好控制的体外细胞系统中的应用。对这些系统的分析应有助于确定各种生物过程和病理条件的特定标志物。
Extracellular vesicles have created great interest as possible source of biomarkers for different biological processes and diseases. Although the biological function of these vesicles is not fully understood, it is clear that they participate in the removal of unnecessary cellular material and act as carriers of various macromolecules and signals between the cells. In this report, we analyzed the proteome of extracellular vesicles secreted by primary hepatocytes. We used one- and two-dimensional liquid chromatography combined with data-independent mass spectrometry. Employing label-free quantitative proteomics, we detected significant changes in vesicle protein expression levels in this in vitro model after exposure to well-known liver toxins (galactosamine and Escherichia coli-derived lipopolysaccharide). The results allowed us to identify candidate markers for liver injury. We validated a number of these markers in vivo, providing the basis for the development of novel methods to evaluate drug toxicity. This report strongly supports the application of proteomics in the study of extracellular vesicles released by well-controlled in vitro cellular systems. Analysis of such systems should help to identify specific markers for various biological processes and pathological conditions.
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影响因子：
3.7
作者：
Jahn H;Wittke S;Zürbig P;Raedler TJ;Arlt S;Kellmann M;Mullen W;Eichenlaub M;Mischak H;Wiedemann K
通讯作者：
Wiedemann K
影响因子：
3.1
作者：
Angeles, Arkhjamil;Fung, Gabriel;Luo, Honglin
通讯作者：
Luo, Honglin
影响因子：
13.5
作者：
Csak, Timea;Ganz, Michal;Pespisa, Justin;Kodys, Karen;Dolganiuc, Angela;Szabo, Gyongyi
通讯作者：
Szabo, Gyongyi
影响因子：
4.8
作者：
Chen, LX;Zeng, Y;Lu, SC
通讯作者：
Lu, SC
影响因子：
6.6
作者：
Hatfield MJ;Potter PM
通讯作者：
Potter PM