Preliminary biochemical characterization of ‘veil’ structure purified from Theileria sergenti-, T. buffeli- and T. orientalis-infected bovine erythrocytes
Preliminary biochemical characterization of ‘veil’ structure purified from Theileria sergenti-, T. buffeli- and T. orientalis-infected bovine erythrocytes
复制标题
从塞氏泰勒虫、布氏泰勒虫和东方泰勒虫感染的牛红细胞中纯化的“面纱”结构的初步生化表征
作者：
C. Sugimoto;S. Kawazu;M. Sato;T. Kamio;K. Fujisaki
在瑟氏泰勒虫（Theileria sergenti-，T. buffeli或T.通过Percoll密度梯度离心从红细胞裂解物中纯化东方蜱感染的牛红细胞。在电子显微镜检查中，面纱由电子致密材料组成，显示出周期性的条纹，并且没有被膜包围。通过一维和二维聚丙烯酰胺凝胶电泳对面纱蛋白的分析表明，面纱含有血红蛋白和其他几种分子量为15·5至46 kDa的碱性蛋白。通过比较的蛋白质模式的面纱与纯化的piroplastin和未感染的牛红细胞，这些碱性蛋白质似乎是寄生虫的起源。这可能是由寄生虫分泌的血红蛋白和蛋白质在红细胞内沉淀形成的面纱。结果表明，T. sergenti，T. buffeli和T.东方
SUMMARY A structure called ‘veil’ in Theileria sergenti-, T. buffeli- or T. orientalis-infected bovine erythrocytes was purified from erythrocyte lysates by Percoll density-gradient centrifugation. On electron microscopical examination, the veils consisted of electron-dense material showing a periodicity of striations and were not surrounded by membranes. Analyses of veil proteins by one- and two-dimensional polyacrylamide gel electrophoresis revealed that the veils contain haemoglobin and several other basic proteins of molecular weight ranging from 15·5 to 46 kDa. By comparing the protein patterns of the veil with those of purified piroplasms and uninfected bovine erythrocytes, these basic proteins appeared to be of parasite origin. It would appear likely that the veils formed by intra-erythrocytic precipitation of haemoglobin and proteins excreted by the parasites. Differences in veil constituents were found between T. sergenti, T. buffeli and T. orientalis.
DOI：
10.1073/pnas.88.2.325
发表时间：
1991-01-01
影响因子：
11.1
作者：
SLATER, AFG;SWIGGARD, WJ;HENDERSON, GB
通讯作者：
HENDERSON, GB