Early decline in c-myb oncogene expression in the differentiation of human myeloblastic leukemia (ML-1) cells induced with 12-O-tetradecanoylphorbol-13-acetate.
Early decline in c-myb oncogene expression in the differentiation of human myeloblastic leukemia (ML-1) cells induced with 12-O-tetradecanoylphorbol-13-acetate.
复制标题
在 12-O-十四烷酰佛波醇-13-乙酸酯诱导的人成髓细胞白血病 (ML-1) 细胞分化过程中，c-myb 癌基因表达的早期下降。
作者：
R. Craig;A. Bloch
癌基因表达与增殖和分化的关系已在人髓性白血病（ML-1）细胞系中进行了研究。发现增殖性白血病细胞表达禽成髓细胞瘤病毒转化序列的4.3-脱氢酶细胞同源物（c-myb）。在用12-O-十四烷酰基佛波醇-13-乙酸酯诱导分化的细胞中观察到该转录物表达的快速下降。早在12-O-十四酰基佛波醇-13-乙酸酯暴露后3小时，c-myb RNA的水平就降低了50%以上，并且在8至72小时，降低大于或等于4倍。在3小时癌基因表达减少后，DNA合成开始下降;到24小时，细胞增殖停止。此时，单核细胞和巨噬细胞样细胞开始出现。这些发现表明，c-myb在ML-1细胞增殖过程中表达，并在伴随分化过程的DNA合成丧失之前下降。
The relationship of oncogene expression to proliferation and differentiation has been examined in a line of human myeloblastic leukemia (ML-1) cells. Proliferating leukemic cells were found to express a 4.3-kilobase cellular homologue (c-myb) of the transforming sequence of avian myeloblastosis virus. A rapid decline in the expression of this transcript was seen in cells induced to differentiate with 12-O-tetradecanoylphorbol-13-acetate. The level of c-myb RNA was decreased by greater than 50% as early as 3 hr after 12-O-tetradecanoylphorbol-13-acetate exposure, and at 8 to 72 hr the reduction was greater than or equal to 4-fold. Subsequent to the decrease in oncogene expression at 3 hr, DNA synthesis began to decline; by 24 hr, cell proliferation had ceased. At this time, monocyte- and macrophage-like cells were beginning to emerge. These findings demonstrate that c-myb is expressed during ML-1 cell proliferation and declines prior to the loss of DNA synthesis that accompanies the differentiation process.
影响因子：
11.2
作者：
D. Rosson;A. Tereba
通讯作者：
D. Rosson;A. Tereba