Cryptomerione induces Th1 cell polarization via influencing IL-10 production by cholera toxin-primed dendritic cells.
Cryptomerione induces Th1 cell polarization via influencing IL-10 production by cholera toxin-primed dendritic cells.
复制标题
柳杉酮通过影响霍乱毒素引发的树突状细胞产生 IL-10 来诱导 Th1 细胞极化。
DOI：
10.1016/j.ejphar.2009.11.031
复制
发表时间：
2010
影响因子：
5
通讯作者：
T. Hashimoto
中科院分区：
文献类型：
--
作者：
M. Takei;A. Umeyama;Je;N. Shoji;T. Hashimoto
树突状细胞在免疫应答的启动和调控中起着重要作用。树突状细胞对naïve T细胞向Th1、Th2或Th17效应细胞的分化具有关键影响。柳杉酮是从柳杉（Cryptomeria japonica）的心材中分离得到的萜类化合物。在这项研究中，我们在体外研究了隐毛蕊酮对人单核细胞来源的树突状细胞表型和功能成熟的影响。将人单核细胞单独暴露于Cryptomerione，或与脂多糖（LPS）或霍乱毒素（CT）联合暴露，然后与naïve T细胞共培养。我们未发现cryptomerione引发的树突状细胞中CD1a、CD80、CD83、CD86和HLA-DR的表达增强。然而，Cryptomerione在异体混合淋巴细胞对ct引发的树突状细胞反应中增强了T细胞的刺激能力，并影响了ct引发的树突状细胞产生白细胞介素(IL)-10和IL-12p70，而对lps引发的树突状细胞没有影响。隐毛利酮还能抑制ct引发的树突状细胞诱导的Th2细胞极化，但能增强naïve T细胞与ct引发的树突状细胞共培养的IFN-γ分泌。用抗il -10单抗和抗ox40l单抗治疗后，ct引发的树突状细胞单独或与Cryptomerione联合产生的细胞因子也受到影响。因此，Cryptomerione诱导的ct启动树突状细胞中Th1极化增强的潜在机制似乎依赖于IL-10分泌和OX40L。这些结果表明Cryptomerione以一种有利于Th1/Th2细胞极化的方式调节人类树突状细胞的功能。
Dendritic cells play an important role in the initiation and regulation of immune response. Dendritic cells have a key influence in the differentiation of naïve T cells into Th1, Th2 or Th17 effector cells. Cryptomerione is terpene isolated from the heartwood of Cryptomeria japonica. In this study, we investigated the effects of Cryptomerione on the phenotypic and functional maturation of human monocyte-derived dendritic cells in vitro. Human monocytes were exposed to either Cryptomerione alone, or in combination with lipopolysaccaride (LPS) or cholera toxin (CT) and thereafter co-cultured with naïve T cells. We found no enhanced CD1a, CD80, CD83, CD86 and HLA-DR expression on Cryptomerione-primed dendritic cells. However, Cryptomerione augmented T cell stimulatory capacity in an allogeneic mixed lymphocyte reaction to CT-primed dendritic cells and influenced the production of interleukin (IL)-10 and IL-12p70 by CT-primed dendritic cells, but not LPS-primed dendritic cells. Cryptomerione also inhibited Th2 cell polarization induced by CT-primed dendritic cells, but enhanced IFN-γ secretion by naïve T cells co-cultured with CT-primed dendritic cells. Cytokine production by CT-primed dendritic cells alone, or in combination with Cryptomerione was also influenced following treatment with anti-IL-10 mAb and anti-OX40L mAb. Thus, the potential mechanisms underlying the enhancement of Th1 polarization in CT-primed dendritic cells induced by Cryptomerione appeared to depend on IL-10 secretion and OX40L. These results suggest that Cryptomerione modulates human dendritic cells function in a fashion that favors Th1/Th2 cell polarization.
影响因子：
56.9
作者：
HSIEH, CS;MACATONIA, SE;MURPHY, KM
通讯作者：
MURPHY, KM