Role of Influenza B virus in hepatic steatosis and mitochondrial abnormalities in a mouse model of reye syndrome
Role of Influenza B virus in hepatic steatosis and mitochondrial abnormalities in a mouse model of reye syndrome
复制标题
乙型流感病毒在雷氏综合征小鼠模型肝脂肪变性和线粒体异常中的作用
作者：
K. Schwarz;S. Larroya;C. Vogler;C. Sippel;Sharon Homan;Ron Cockrell;I. Schulze
在Reye综合征的B型流感病毒小鼠模型中观察到的肝脏脂肪变性归因于感染性病毒，或者归因于病毒处理小鼠的摄食量减少或病毒制剂中的杂质。为了解决这个问题，通过静脉注射给予4 - 6周龄雄性BalB C小鼠12，800血凝单位的流感B Lee/40病毒的磷酸盐缓冲盐水/1%牛血清白蛋白溶液，使用通过超离心从感染的尿囊液制备的病毒，通过蔗糖密度梯度纯化通过超离心从感染的尿囊液制备的病毒或通过照射通过以下方法制备的病毒从感染的尿囊液中超离心分离病毒。通过超离心从感染的尿囊液制备的病毒的感染性滴度远高于从感染的尿囊液制备的蔗糖密度梯度纯化病毒的感染性滴度：用超浓缩法制备的病毒的50%鸡胚感染剂量为3.9 × 104/血凝单位，而用超浓缩法制备的病毒的50%鸡胚感染剂量为8.7 × 104/血凝单位。从感染的尿囊液中制备的蔗糖密度梯度纯化病毒的血凝单位。对照小鼠接受磷酸盐缓冲盐水/1%牛血清白蛋白或用磷酸盐缓冲盐水/1%牛血清白蛋白稀释的未感染尿囊液。小鼠禁食以消除饮食变化，并在病毒接种后36小时获得肝脏。在上述治疗中，与对照组相比，只有通过从感染的尿囊液中超离心制备的病毒引起临床疾病和肝甘油三酯增加（p < 0.02）。从感染的尿囊液中通过超离心制备的病毒中的肝甘油三酯与组织病理学空泡化评分相关（r = 0.5773; p < 0.03）。
The hepatic steatosis observed in the influenza B virus mouse model of Reye syndrome has been attributed to infectious virus or, alternately, to decreased food intake in the virus‐treated mice or impurities in the virus preparation. To resolve this issue, 4‐ to 6‐wk‐old male Balb C mice were given, by intravenous injection, 12,800 hemagglutination units of influenza B Lee/40 virus in phosphate buffered saline/1% bovine serum albumin using virus prepared by ultracentrifugation from infected allantoic fluid, by sucrose density‐gradient purification of virus prepared by ultracentrifugation from infected allantoic fluid or by irradiation of virus prepared by ultracentrifugation from infected allantoic fluid to inactivate virus. The infectivity titer of virus prepared by ultracentrifugation from infected allantoic fluid was much higher than that of sucrose density‐gradient purified virus prepared from infected allantoic fluid: 50% egg infectious dose for virus prepared by ultracentrifugation from infected allantoic fluid was 3.9 × 104/hemagglutination unit vs. 8.7 50% egg infectious dose/hemagglutination unit for sucrose density‐gradient purified virus prepared from infected allantoic fluid. Control mice received phosphate‐buffered saline/1% bovine serum albumin or uninfected allantoic fluid diluted in phosphate‐buffered saline/1% bovine serum albumin. Mice were fasted to eliminate dietary variation, and livers were obtained 36 hr after virus administration. Of the above treatments, only virus prepared by ultracentrifugation from infected allantoic fluid caused clinical illness and increased hepatic triglycerides (p < 0.02) compared with controls. Hepatic triglycerides in virus prepared by ultracentrifugation from infected allantoic fluid correlated with histopathological vacuolization scores (r = 0.5773; p < 0.03).
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DOI：
10.1016/s0021-9258(17)43782-3
发表时间：
1983-12
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Y. Lange;B. Ramos
通讯作者：
Y. Lange;B. Ramos
四氯化碳和三氯溴甲烷破坏肝微粒体钙泵活性。
影响因子：
5.8
作者：
Lowrey,K;GlendeJr,EA;Recknagel,RO
通讯作者：
Recknagel,RO
雷氏综合征中的尿二羧酸。
DOI：
10.1016/s0022-3476(85)80619-3
发表时间：
1985
期刊：
The Journal of pediatrics
影响因子：
--
作者：
Tonsgard,JH
通讯作者：
Tonsgard,JH
影响因子：
15.9
作者：
BACON, BR;TAVILL, AS;RECKNAGEL, RO
通讯作者：
RECKNAGEL, RO
影响因子：
5.8
作者：
JORDAN, RA;SCHENKMAN, JB
通讯作者：
SCHENKMAN, JB