Filter characteristics influencing circulating tumor cell enrichment from whole blood.
Filter characteristics influencing circulating tumor cell enrichment from whole blood.
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DOI：
10.1371/journal.pone.0061770
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Terstappen LW
中科院分区：
文献类型：
--
作者：
Coumans FA;van Dalum G;Beck M;Terstappen LW
根据血细胞和 CTC 物理特性的差异，已经描述了多种过滤测定法来富集循环肿瘤细胞 (CTC)。在本研究中，我们评估了不同的过滤器类型，以获得 CTC 富集的理想过滤器的特性。将掺有肿瘤细胞系细胞的 0.1 至 10 mL 全血通过氮化硅微筛、聚合物径迹蚀刻过滤器和具有不同孔径的金属 TEM 网格。确定了 9 种不同培养细胞系的恢复情况和大小，并将其与转移性乳腺癌、结直肠癌和前列腺癌患者的 EpCAM+CK+CD45−DNA+ CTC 的大小进行比较。 8 µm 径迹蚀刻过滤器和 5 µm 微筛对 MDA-231、PC3-9 和 SKBR-3 细胞具有最佳性能，富集全血中 >80% 的细胞。 TEM 网格的回收率很差，约为 25%。细胞系的中位直径范围为 10.9–19.0 µm，而乳腺癌、前列腺和结直肠 CTC 的中位直径分别为 13.1、10.7 和 11.0 µm。 11.4 µm COLO-320 细胞系的回收率最低，为 17%。用于 CTC 富集的理想过滤器由坚硬、平坦的材料制成，对血细胞呈惰性，具有至少 100,000 个规则间隔的 5 µm 孔，可容纳 1 ml 血液，孔隙率 ≤10%。虽然细胞大小是决定恢复的重要因素，但还必须涉及其他因素。为了评估过滤程序，应使用中值大小为 11-13 µm 的细胞系来挑战系统。
A variety of filters assays have been described to enrich circulating tumor cells (CTC) based on differences in physical characteristics of blood cells and CTC. In this study we evaluate different filter types to derive the properties of the ideal filter for CTC enrichment. Between 0.1 and 10 mL of whole blood spiked with cells from tumor cell lines were passed through silicon nitride microsieves, polymer track-etched filters and metal TEM grids with various pore sizes. The recovery and size of 9 different culture cell lines was determined and compared to the size of EpCAM+CK+CD45−DNA+ CTC from patients with metastatic breast, colorectal and prostate cancer. The 8 µm track-etched filter and the 5 µm microsieve had the best performance on MDA-231, PC3-9 and SKBR-3 cells, enriching >80% of cells from whole blood. TEM grids had poor recovery of ∼25%. Median diameter of cell lines ranged from 10.9–19.0 µm, compared to 13.1, 10.7, and 11.0 µm for breast, prostate and colorectal CTC, respectively. The 11.4 µm COLO-320 cell line had the lowest recovery of 17%. The ideal filter for CTC enrichment is constructed of a stiff, flat material, is inert to blood cells, has at least 100,000 regularly spaced 5 µm pores for 1 ml of blood with a ≤10% porosity. While cell size is an important factor in determining recovery, other factors must be involved as well. To evaluate a filtration procedure, cell lines with a median size of 11–13 µm should be used to challenge the system.
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影响因子：
158.5
作者：
Cristofanilli, M;Budd, GT;Hayes, DF
通讯作者：
Hayes, DF
DOI：
10.1158/1078-0432.ccr-10-1105
发表时间：
2010-10-15
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Lin HK;Zheng S;Williams AJ;Balic M;Groshen S;Scher HI;Fleisher M;Stadler W;Datar RH;Tai YC;Cote RJ
通讯作者：
Cote RJ
影响因子：
50.5
作者：
Coumans, F. A. W.;Doggen, C. J. M.;Terstappen, L. W. M. M.
通讯作者：
Terstappen, L. W. M. M.
影响因子：
50.5
作者：
Hiltermann, T. J. N.;Pore, M. M.;Groen, H. J. M.
通讯作者：
Groen, H. J. M.
影响因子：
12.6
作者：
Hsieh, HB;Marrinucci, D;Nieva, J
通讯作者：
Nieva, J