Gradient/Extremely Low-Frequency Magnetic Field Affects Metamorphic Behaviors in Thyroxine-Administrated Axolotls: Regulation of Amphibian Metamorphosis Depending on Field Strength and Exposure Timing
Gradient/Extremely Low-Frequency Magnetic Field Affects Metamorphic Behaviors in Thyroxine-Administrated Axolotls: Regulation of Amphibian Metamorphosis Depending on Field Strength and Exposure Timing
复制标题
梯度/极低频磁场影响甲状腺素施用蝾螈的变态行为：根据场强和暴露时间调节两栖动物变态
DOI：
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发表时间：
2018
影响因子：
2.1
通讯作者：
M. Ohuchi
中科院分区：
文献类型：
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作者：
H. Nakagawa;M. Ohuchi
在本文中，我们研究了梯度磁场或极低频（ELF）场对甲状腺激素诱导的墨西哥蝾螈强制变态的影响。在蝾螈适应我们的实验环境后，将它们单独保存在 L-甲状腺素 (T4) 溶液中，暴露于 17 T/m 的梯度场或 10 Hz 1.0-5.0 mT 的 ELF 场。为了消除蝾螈不必要的压力，我们最近开发了一种原创的水更新系统，配备虹吸、速度和温度控制。在 5.0 mT ELF 暴露下，完美变态为蝾螈所需的天数平均为 16.9 天，与对照的时间段（15.1 天）相反。我们假设，暴露于 ELF 的蝾螈的形态延迟高达 26%，可能是由于蝾螈受试者体内交变场效应和对磁力的生物适应性反应之间的竞争情况造成的。此外，我们确实发现梯度场暴露的启动时间确实会影响以下蝾螈的存活率。在形态学完成时间点之前暴露的情况下，速率的下降是明显的。我们的数据极大地支持了这样的观点，即磁场暴露可能会细微地改变蝾螈的变态，具体取决于暴露时间、磁场强度、频率等。我们强烈建议研究甲状腺激素诱导的蝾螈变态的磁控制。
In this paper, we investigated the influences of a gradient magnetic field or extremely low-frequency (ELF) fields on the forced metamorphosis of the Mexican axolotls, which was induced with a thyroid hormone. After the adaptation of the axolotls to our experimental environment, they were individually kept in an L-thyroxine (T4) solution under exposure to a gradient field of 17 T/m or ELF fields of 1.0–5.0 mT at 10 Hz. In order to remove unnecessary stress for the axolotls, we recently developed an original water-renewing system equipped with siphonage, velocity, and temperature controls. The number of days required for the perfect metamorphosis into salamanded axolotls under the ELF exposure of 5.0 mT was 16.9 days on average, contrary to the time period for a control (15.1 days). We assumed that the morphological delays of up to 26% among the ELF-exposed axolotls might be due to the competing situation between alternating field effects and biological adaptive responses to the magnetic forces in the axolotl subjects’ bodies. In addition, we did found that the initiation timings of the gradient-field exposure did affect the survival rates of the following salamanded axolotls. A decrease in the rates was noticeable in the case of an exposure just before the time point of the morphological completion. Our data greatly support the idea that magnetic field exposures might modify axolotl metamorphosis minutely, depending on the exposure timing, the field strength, the frequency, and so on. We strongly propose investigating the magnetic control of axolotl metamorphosis induced with a thyroid hormone.
影响因子：
2.7
作者：
Page, Robert B.;Monaghan, James R.;Walker, John A.;Voss, S. Randal
通讯作者：
Voss, S. Randal