Altered PTEN expression; a diagnostic marker for differentiating normal, hyperplastic and neoplastic endometrium.
Altered PTEN expression; a diagnostic marker for differentiating normal, hyperplastic and neoplastic endometrium.
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DOI：
10.1186/1746-1596-4-41
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发表时间：
2009-11-25
影响因子：
2.6
通讯作者：
Tavangar SM
中科院分区：
文献类型：
--
作者：
Sarmadi S;Izadi-Mood N;Sotoudeh K;Tavangar SM
不同的分子改变已被描述在子宫内膜样癌（EECA）。其中最常见的改变是肿瘤抑制基因PTEN蛋白的丢失。本研究旨在探讨PTEN基因在正常子宫内膜、增生性子宫内膜和肿瘤性子宫内膜中的表达情况。在伊朗德黑兰的一家转诊妇科医院的一项研究中，对以下三组的87个连续标本进行了PTEN的免疫组织化学（IHC）评价：A组-正常增殖性子宫内膜（n = 29）; B组-增生性子宫内膜[包括无子宫内膜异位症的简单增生（n = 21）和有子宫内膜异位症的复杂增生（n = 8）]和C组-EECA（n = 29）。根据玻片染色面积和颜色反应强度，采用任意定量方法分析细胞免疫染色。PTEN在正常增生期子宫内膜、单纯性增生、不典型复杂性增生和EECA中的阳性率分别为75%和48%（P <0.001）。子宫内膜增生症组PTEN蛋白表达强度明显高于增生症组和EECA组（P <0.001）。PTEN在周期性子宫内膜中的表达明显高于不典型增生和子宫内膜样癌。
Different molecular alterations have been described in endometrioid endometrial carcinoma (EECA). Among them the most frequently altered is loss of the PTEN protein, a tumor suppressor gene. The purpose of this study was to evaluate the expression pattern of PTEN gene in normal, hyperplastic and neoplastic endometrium. In a study in a referral gynecologic hospital in Tehran, Iran, immunohistochemical (IHC) evaluation of PTEN was performed on 87 consecutive specimens to the following three groups; group A- normal proliferative endometrium(n = 29); group B- hyperplastic endometrium [including simple hyperplasia without atypia(n = 21) and complex hyperplasia with atypia (n = 8)] and group C- EECA(n = 29). Immunostaining of cells was analyzed by arbitrary quantitative methods according to both slide's area staining and intensity of color reaction. PTEN immunoreactivity was present in all normal proliferative endometrium, all simple hyperplasia, 75% of atypical complex hyperplasia and in 48% of EECA (P < 0.001). The intensity of PTEN reaction was significantly higher in group with proliferative endometrium than hyperplastic endometrium and EECA (P < 0.001). PTEN expression was significantly higher in cyclical endometrium than in atypical hyperplasia and endometrioid carcinoma.
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影响因子：
2.8
作者：
Kapucuoglu, Nilgun;Aktepe, Fatma;Ciris, Metin
通讯作者：
Ciris, Metin
影响因子：
6.2
作者：
Salvesen, HB;Stefansson, I;Akslen, LA
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影响因子：
11.2
作者：
Lacey JV Jr;Mutter GL;Ronnett BM;Ioffe OB;Duggan MA;Rush BB;Glass AG;Richesson DA;Chatterjee N;Langholz B;Sherman ME
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Sherman ME
影响因子：
2.4
作者：
Boruban, Melih C.;Altundag, Kadri;Blankstein, Josef
通讯作者：
Blankstein, Josef
影响因子：
3.3
作者：
Baak, JPA;van Diermen, B;Lovslett, K
通讯作者：
Lovslett, K