Cloning of cDNA encoding vitellogenin and its expression in red sea urchin, Pseudocentrotus depressus
Cloning of cDNA encoding vitellogenin and its expression in red sea urchin, Pseudocentrotus depressus
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卵黄原蛋白cDNA的克隆及其在红海胆中的表达
DOI：
10.2108/zsj.18.559
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
K. Mori
中科院分区：
文献类型：
--
作者：
T. Unuma;H. Okamoto;K. Konishi;H. Ohta;K. Mori
摘要在雄、雌红海胆配子发生开始前，其未成熟性腺的营养吞噬细胞中积累了大量的主要卵黄蛋白（MYP）。为了研究这种蛋白质在性腺中的积累机制，我们克隆了全长cDNA编码卵黄蛋白原（Vg的MYP前体在体腔液），并研究了其在各种组织中的未成熟的成年人的表达。其核苷酸序列包含一个4050 bp的开放阅读框，编码1349个氨基酸。推导的N端氨基酸序列与脊椎动物转铁蛋白家族的氨基酸序列同源性为25%。北方印迹分析表明，卵黄蛋白原mRNA在卵巢、精巢、胃、肠和直肠中均有表达，在性腺中表达量最高。RT-PCR检测到食管和体腔细胞中也有弱表达。原位杂交结果表明，营养吞噬细胞，专门填补管腔的未成熟的性腺，含有Vg mRNA。这些结果表明，储存在未成熟性腺中的MYP主要在营养性吞噬细胞中合成和积累。
Abstract Both male and female red sea urchins, Pseudocentrotus depressus, accumulate a large quantity of the major yolk protein (MYP) in the nutritive phagocytes of immature gonads before the initiation of game-togenesis. To examine the accumulation mechanism of this protein in the gonad, we cloned full-length cDNA encoding vitellogenin (Vg; the MYP precursor in the coelomic fluid), and investigated its expression in various tissues of immature adults. The nucleotide sequence of Vg contains an open reading frame of 4050 bp encoding 1349 amino acids. The deduced amino acid sequence near the N-terminal showed 25% homology to the vertebrate transferrin family. Vitellogenin mRNA was detected in the ovary, testis, stomach, intestine and rectum by Northern blot analysis, with the highest level of mRNA expression in the gonad. Weak expression was also detected in the esophagus and coelomocytes by RT-PCR. In situ hybridization demonstrated that nutritive phagocytes, which exclusively fill the lumina of the immature gonad, contained Vg mRNA. These results suggested that the MYP stored in the immature gonads is synthesized and accumulated mainly within the nutritive phagocytes.
海胆 Strongylocentrotus purpuratus 中编码卵黄蛋白原的单个基因：5 端序列。
影响因子：
14.9
作者：
Shyu,AB;Blumenthal,T;Raff,RA
通讯作者：
Raff,RA