Three redescriptions in Tintinnopsis (Protista: Ciliophora: Tintinnina) from coastal waters of China, with cytology and phylogenetic analyses based on ribosomal RNA genes.
Three redescriptions in Tintinnopsis (Protista: Ciliophora: Tintinnina) from coastal waters of China, with cytology and phylogenetic analyses based on ribosomal RNA genes.
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基于核糖体 RNA 基因的细胞学和系统发育分析，对中国沿海水域的 Tintinnopsis (Protista: Ciliophora: Tintinnina) 进行了三种重新描述
DOI：
10.1186/s12866-020-02057-2
复制
发表时间：
2020-12-14
期刊：
影响因子：
4.2
通讯作者：
Hu X
中科院分区：
文献类型：
--
作者：
Bai Y;Wang R;Song W;Li L;Santoferrara LF;Hu X
背景
丁宁纤毛虫的分类学尚未得到解决，因为它传统上是基于 lorica（一种分泌性外壳），并且最近才纳入细胞学和分子信息。 Tintinnopsis 是物种最丰富的丁丁宁属，也是最有问题的：众所周知，它是非单系的，但在用现代方法研究更多其物种之前，无法对其进行修改。
结果
这里对来自中国沿海水域的 T. hemispiralis Yin (1956)、T. kiaochowensis Yin (1956) 和 T. uruguayensis Balech (1948) 进行了研究。在活体样本和普罗特戈染色样本中对 Lorica 和细胞特征进行了形态学研究，并对三个核糖体 RNA (rRNA) 基因座的序列进行了系统发育分析。这三个物种表现出复杂的纤毛模式（具有腹侧、背侧和后侧运动以及右、左和外侧纤毛场），但在 lorica 形态、体细胞纤毛细节和 rRNA 基因序列方面有所不同。 Tintinnopsis hemispiralis 的进一步特征是纤毛簇（一条非常长的纤毛带，起源于腹侧运动中部并延伸出 lorica）和多个大核结节。胶州T. kiaochowensis和T. uruguayensis都有两个大核结节，但体细胞运动节的数量和后运动节的位置不同。固定T. hemispiralis和T. kiaochowensis两个新型是为了客观地稳定种名，主要是因为之前没有模式材料。通过系统发育分析和与密切相关物种的比较，我们推断纤毛簇和细节（例如外侧纤毛区最右侧运动的开始）是共源性，可能有助于阐明类似丁丁属类群的系统学。
结论
对三种鲜为人知的Tintinnopsis物种的重新描述，即T. hemispiralis、T. kiaochowensis和T. uruguayensis，首次揭示了它们的纤毛模式和rRNA序列。这项研究扩展了丁丁宁的知识和数据库，并有助于识别未来分类重排的潜在共源性。
Background
The taxonomy of tintinnine ciliates is vastly unresolved because it has traditionally been based on the lorica (a secreted shell) and it has only recently incorporated cytological and molecular information. Tintinnopsis, the most speciose tintinnine genus, is also the most problematic: it is known to be non-monophyletic, but it cannot be revised until more of its species are studied with modern methods.
Results
Here, T. hemispiralis Yin, 1956, T. kiaochowensis Yin, 1956, and T. uruguayensis Balech, 1948, from coastal waters of China, were studied. Lorica and cell features were morphometrically investigated in living and protargol-stained specimens, and sequences of three ribosomal RNA (rRNA) loci were phylogenetically analyzed. The three species show a complex ciliary pattern (with ventral, dorsal, and posterior kineties and right, left, and lateral ciliary fields), but differ in lorica morphology, details of the somatic ciliature and rRNA gene sequences. Tintinnopsis hemispiralis is further distinguished by a ciliary tuft (a ribbon of very long cilia originated from the middle portion of the ventral kinety and extending out of the lorica) and multiple macronuclear nodules. Both T. kiaochowensis and T. uruguayensis have two macronuclear nodules, but differ in the number of somatic kineties and the position of the posterior kinety. Two neotypes are fixed for T. hemispiralis and T. kiaochowensis to stabilize the species names objectively, mainly because of the previous unavailability of type materials. By phylogenetic analysis and comparison with closely-related species, we infer that the ciliary tuft and details such as the commencement of the rightmost kinety in the lateral ciliary field are synapomorphies that may help clarify the systematics of Tintinnopsis-like taxa.
Conclusion
The redescriptions of three poorly known Tintinnopsis species, namely T. hemispiralis, T. kiaochowensis, and T. uruguayensis firstly revealed their ciliary patterns and rRNA sequences. This study expands knowledge and database of tintinnines and helps in identifying potential synapomorphies for future taxonomic rearrangements.
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DOI：
10.1111/j.1550-7408.2008.00309.x
发表时间：
2008-03
期刊：
The Journal of eukaryotic microbiology
影响因子：
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作者：
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DOI：
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影响因子：
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