Characterization of sensory neuronal subtypes innervating mouse tongue.
Characterization of sensory neuronal subtypes innervating mouse tongue.
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DOI：
10.1371/journal.pone.0207069
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发表时间：
2018
期刊：
影响因子：
3.7
通讯作者：
Ruparel S
中科院分区：
文献类型：
--
作者：
Wu P;Arris D;Grayson M;Hung CN;Ruparel S
舌头是唯一暴露于水溶性环境化学物质，可能导致损伤或肿瘤发生。然而，相对较少的研究集中在分子和功能组织的三叉神经节（TG）传入神经元支配的舌头。目前的研究确定和表征舌感觉神经元的神经元亚型分类的基础上，以前的特征在于背根神经节（DRG）神经元。我们采用免疫组化转基因报告小鼠系以及单细胞PCR的已知标记的神经元亚型的特征神经元亚型支配的舌头。评价在逆行标记的TG神经元中表达的标记物的表达每种标记物的神经元的比例、表达强度和重叠基因。结果发现，支配舌神经的感觉神经元主要表达CGRP、TRPV 1、TrkC、5 HT 3A和Parvalbumin。这些标记物对应于肽能和非肽能C-伤害感受器、肽能A伤害感受器、本体感受器和有髓鞘低阈值机械感受器（LTMR）的亚组。有趣的是，如前所述，我们还发现了TG和DRG神经元之间的几个差异，表明需要基于所有TG和DRG神经元内的组织类型对神经元类型进行单细胞测序。
The tongue is uniquely exposed to water-soluble environmental chemicals that may lead to injury or tumorigenesis. However, comparatively little research has focused on the molecular and functional organization of trigeminal ganglia (TG) afferent neurons innervating the tongue. The current study identified and characterized lingual sensory neurons based on a neuronal subtype classification previously characterized in the dorsal root ganglion (DRG) neurons. We employed immunohistochemistry on transgenic reporter mouse lines as well as single-cell PCR of known markers of neuronal subtypes to characterize neuronal subtypes innervating the tongue. Markers expressed in retrogradely labeled TG neurons were evaluated for the proportion of neurons expressing each marker, intensity of expression, and overlapping genes. We found that tongue-innervating sensory neurons primarily expressed CGRP, TRPV1, TrkC, 5HT3A and Parvalbumin. These markers correspond to peptidergic and a subgroup of non-peptidergic C-nociceptors, peptidergic A nociceptors, proprioceptors and myelinated low-threshold mechanoreceptors (LTMRs). Interestingly, as reported previously, we also found several differences between TG and DRG neurons indicating the need for single-cell sequencing of neuronal types based on tissue type within all TG as well as DRG neurons.
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DOI：
10.1016/j.jpain.2009.09.008
发表时间：
2010-04
期刊：
The journal of pain
影响因子：
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作者：
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发表时间：
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期刊：
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作者：
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