Clenbuterol prevents epinephrine from antagonizing insulin-stimulated muscle glucose uptake.
Clenbuterol prevents epinephrine from antagonizing insulin-stimulated muscle glucose uptake.
复制标题
克仑特罗可防止肾上腺素拮抗胰岛素刺激的肌肉葡萄糖摄取。
作者：
D. Hunt;Z. Ding;J. Ivy
在本研究中，我们研究了在离体大鼠骨骼肌中存在肾上腺素的情况下，慢性克伦特罗治疗对胰岛素刺激的葡萄糖摄取的影响。胰岛素（50 microU/ml）增加快肌（滑车上肌）和慢肌（比目鱼肌）的葡萄糖摄取。在 24 nM 肾上腺素存在下，胰岛素刺激的葡萄糖摄取被完全抑制。肾上腺素对葡萄糖摄取的抑制伴随着细胞内葡萄糖 6-磷酸浓度的增加和胰岛素受体底物 1 相关磷脂酰肌醇 3-激酶 (IRS-1/PI3-激酶) 活性的降低。克伦特罗治疗对胰岛素刺激的葡萄糖摄取没有直接影响。然而，在克仑特罗治疗后，肾上腺素无法有效减弱胰岛素刺激的肌肉葡萄糖摄取。肾上腺素无法有效抑制胰岛素刺激的葡萄糖摄取，同时也无法增加细胞内 6-磷酸葡萄糖的浓度并减弱 IRS-1/PI3-激酶活性。这项研究的结果表明，在用克仑特罗预处理的大鼠的肌肉中，肾上腺素抑制胰岛素刺激的葡萄糖摄取的有效性严重减弱。
In the present study, we investigated the effects of chronic clenbuterol treatment on insulin-stimulated glucose uptake in the presence of epinephrine in isolated rat skeletal muscle. Insulin (50 microU/ml) increased glucose uptake in both fast-twitch (epitrochlearis) and slow-twitch (soleus) muscles. In the presence of 24 nM epinephrine, insulin-stimulated glucose uptake was completely suppressed. This suppression of glucose uptake by epinephrine was accompanied by an increase in the intracellular concentration of glucose 6-phosphate and a decrease in insulin-receptor substrate-1-associated phosphatidylinositol 3-kinase (IRS-1/PI3-kinase) activity. Clenbuterol treatment had no direct effect on insulin-stimulated glucose uptake. However, after clenbuterol treatment, epinephrine was ineffective in attenuating insulin-stimulated muscle glucose uptake. This ineffectiveness of epinephrine to suppress insulin-stimulated glucose uptake occurred in conjunction with its inability to increase the intracellular concentration of glucose 6-phosphate and attenuate IRS-1/PI3-kinase activity. Results of this study indicate that the effectiveness of epinephrine to inhibit insulin-stimulated glucose uptake is severely diminished in muscle from rats pretreated with clenbuterol.
脂肪细胞中β-肾上腺素能受体的脱敏导致葡萄糖转运的胰岛素敏感性增加。
DOI：
10.1152/ajpendo.1996.271.2.e271
发表时间：
1996
期刊：
The American journal of physiology.
影响因子：
--
作者：
Green,A;Carroll,RM;Dobias,SB
通讯作者：
Dobias,SB
增加 IM-9 细胞的 cAMP 含量会改变胰岛素受体的磷酸化状态和蛋白激酶活性。
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Stadtmauer,L;Rosen,OM
通讯作者：
Rosen,OM
影响因子：
3.3
作者：
MESZAROS, K;LANG, CH;SPITZER, JJ
通讯作者：
SPITZER, JJ