Human papillomavirus 16 infection in adenocarcinoma of the cervix.
Human papillomavirus 16 infection in adenocarcinoma of the cervix.
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DOI：
10.1038/sj.bjc.6602855
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发表时间：
2005-11-28
影响因子：
8.8
通讯作者：
中科院分区：
文献类型：
--
作者：
有组织的宫颈筛查计划的成功带来的影响是，宫颈鳞状细胞癌的发病率稳步下降，但随之而来的是不常见的组织学亚型的发病率上升，特别是子宫颈腺癌(ACC)。虽然人类乳头瘤病毒(HPV)感染被认为是宫颈癌的必要原因，但它在ACC发病机制中的作用尚未得到很好的证实。HPV致癌株和ACC之间已建立的联系是基于对整个肿瘤切片进行的分子研究。在这项研究中，使用PixCell II激光显微切割系统对一组10年来被诊断为ACC的女性的子宫腺癌细胞进行解剖，利用实时定量聚合酶链式反应检测HPV16基因组序列，以确认ACC细胞中存在HPVDNA。通过结合这两项复杂的技术，可以计算出HPV DNA拷贝数细胞，以研究其作用。该队列中HPV16感染率为24%，显著高于对照组(χ2，P=0.014)。慢性萎缩性胃炎患者的每细胞HPVDNA拷贝数也显著高于对照组(P=0.00007)。HPV DNA拷贝数越高，发生ACC的风险越大。
The impact of the success of organised cervical screening programme results in a steady decline of the incidence of squamous cell carcinoma of the cervix but a concomitant increase in the incidence of the less common histological subtypes, particularly adenocarcinoma of the cervix (ACC). Although Human papillomavirus (HPV) infection is believed to be a necessary cause of cervical cancer, its role in the pathogenesis of ACC is not well established. Established associations between oncogenic strains of HPV and ACC are based on molecular studies carried out on entire tumour block sections. In this study, the cervical adenocarcinoma cells of a 10-year cohort of women diagnosed with ACC were dissected using the PixCell II Laser Microdissecting System to detect the HPV 16 genome sequence using the real-time quantitative polymerase chain reaction to confirm the presence of HPV DNA within ACC cells. By coupling these two sophisticated techniques, the HPV DNA copy number cell could be calculated to investigate its role. The prevalence of HPV 16 infection in this cohort was 24%, which is significantly higher than the control group (χ2, P=0.014). Women with ACC also had significantly higher HPV DNA copy number per cell compared to the control group (P=0.00007). Higher HPV DNA copy number is associated with risk of developing ACC.
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影响因子：
3.8
作者：
Meissner, JD
通讯作者：
Meissner, JD
DOI：
10.1053/beog.2001.0213
发表时间：
2001-10-01
影响因子：
5.5
作者：
Stanley, MA
通讯作者：
Stanley, MA
影响因子：
168.9
作者：
Sasieni, P;Adams, J
通讯作者：
Adams, J
影响因子：
10.3
作者：
SCHIFFMAN, MH
通讯作者：
SCHIFFMAN, MH
影响因子：
168.9
作者：
Ylitalo, N;Sorensen, P;Melbye, M
通讯作者：
Melbye, M