Phenotypic plasticity of mouse spermatogonial stem cells.
Phenotypic plasticity of mouse spermatogonial stem cells.
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DOI：
10.1371/journal.pone.0007909
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发表时间：
2009-11-19
期刊：
影响因子：
3.7
通讯作者：
Shinohara T
中科院分区：
文献类型：
--
作者：
Morimoto H;Kanatsu-Shinohara M;Takashima S;Chuma S;Nakatsuji N;Takehashi M;Shinohara T
精原干细胞（spermatogonialstemcells，SSCs）通过不断的自我更新分裂来支持精子发生。SSC被认为具有固定的表型，生殖细胞移植技术的发展促进了它们的表征和以确定性方式的前瞻性分离;然而，我们的体外SSC培养实验表明培养细胞的异质性，并表明它们可能不遵循体外确定性命运承诺。在这项研究中，我们报告的表型可塑性的精原干细胞。虽然c-kit酪氨酸激酶受体（Kit）在体内的SSC中不表达，但当SSC在体外层粘连蛋白上培养时，它被上调。培养中的Kit-和Kit+细胞在生殖细胞移植后显示出相当水平的SSC活性。与依赖Kit生存和增殖的精原细胞分化不同，SSC上表达的Kit在SSC自我更新中不起任何作用。此外，在体内生殖细胞移植后，一旦开始增殖，精原干细胞上的Kit表达就动态变化。这些结果表明，精原干细胞可以根据其微环境和随机表达试剂盒改变其表型。我们的研究结果还表明，活化和非活化的精原干细胞表现出不同的表型。
Spermatogonial stem cells (SSCs) continuously undergo self-renewal division to support spermatogenesis. SSCs are thought to have a fixed phenotype, and development of a germ cell transplantation technique facilitated their characterization and prospective isolation in a deterministic manner; however, our in vitro SSC culture experiments indicated heterogeneity of cultured cells and suggested that they might not follow deterministic fate commitment in vitro. In this study, we report phenotypic plasticity of SSCs. Although c-kit tyrosine kinase receptor (Kit) is not expressed in SSCs in vivo, it was upregulated when SSCs were cultured on laminin in vitro. Both Kit− and Kit+ cells in culture showed comparable levels of SSC activity after germ cell transplantation. Unlike differentiating spermatogonia that depend on Kit for survival and proliferation, Kit expressed on SSCs did not play any role in SSC self-renewal. Moreover, Kit expression on SSCs changed dynamically once proliferation began after germ cell transplantation in vivo. These results indicate that SSCs can change their phenotype according to their microenvironment and stochastically express Kit. Our results also suggest that activated and non-activated SSCs show distinct phenotypes.
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DOI：
10.1073/pnas.0508780102
发表时间：
2005-11-29
影响因子：
11.1
作者：
Hamra, FK;Chapman, KM;Garbers, DL
通讯作者：
Garbers, DL
DOI：
10.1073/pnas.88.11.4671
发表时间：
1991-06-01
影响因子：
11.1
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
3.6
作者：
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通讯作者：
Shinohara, T
影响因子：
3.6
作者：
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通讯作者：
Shinohara, Takashi