Role of Bacterial Exopolysaccharides (EPS) in the Fate of the Oil Released during the Deepwater Horizon Oil Spill.
Role of Bacterial Exopolysaccharides (EPS) in the Fate of the Oil Released during the Deepwater Horizon Oil Spill.
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DOI：
10.1371/journal.pone.0067717
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Aitken MD
中科院分区：
文献类型：
--
作者：
Gutierrez T;Berry D;Yang T;Mishamandani S;McKay L;Teske A;Aitken MD
盐单胞菌是公认的产生胞外多糖(EPS)的菌种，这些EPS具有两亲性，允许这些大分子与疏水底物(如碳氢化合物)接触。然而，关于嗜盐单胞菌EPS影响碳氢化合物生物降解的潜力的知识仍然很少。在这项研究中，盐生单胞菌TG39产生的具有良好特性的两亲性EPS被证明有效地增加了芳香烃的增溶作用，并增强了在深水地平线漏油活跃期收集的受石油污染的表层水中的本地微生物群落对芳香烃的生物降解作用。从深水地平线分离到3株产EPS的盐生单胞菌，它们都具有优良的乳化性质，与菌株TG39和其他产EPS的盐生单胞菌的16S rRNA序列具有很高的同源性(97-100%)。对泄漏过程中采集的地表水样品的热解序列数据进行分析，发现了几种不同的盐单胞菌系统类型，其中一些与菌株TG39和墨西哥湾泄漏分离株有很高的序列同源性(≥97%)。其他由具有良好特征的EPS产生性质的成员组成的细菌群，如Alteromonas，Colwell lia和PseudoAlternomonas，也被发现富含在地表水中，这表明漏油期间墨西哥湾EPS的总库可能得到了这些微生物的补充。滚筒培养瓶与来自深水地平线泄漏现场的一种卤素单胞菌分离株进行了培养，证明了其有效地产生石油聚集体和乳化石油的能力。在漏油过程中产生EPS的细菌的丰富，加上它们产生两亲性EPS的能力，很可能有助于最终清除石油并形成石油聚集体，这是在受污染的地表水中观察到的主要特征。
Halomonas species are recognized for producing exopolysaccharides (EPS) exhibiting amphiphilic properties that allow these macromolecules to interface with hydrophobic substrates, such as hydrocarbons. There remains a paucity of knowledge, however, on the potential of Halomonas EPS to influence the biodegradation of hydrocarbons. In this study, the well-characterized amphiphilic EPS produced by Halomonas species strain TG39 was shown to effectively increase the solubilization of aromatic hydrocarbons and enhance their biodegradation by an indigenous microbial community from oil-contaminated surface waters collected during the active phase of the Deepwater Horizon oil spill. Three Halomonas strains were isolated from the Deepwater Horizon site, all of which produced EPS with excellent emulsifying qualities and shared high (97-100%) 16S rRNA sequence identity with strain TG39 and other EPS-producing Halomonas strains. Analysis of pyrosequence data from surface water samples collected during the spill revealed several distinct Halomonas phylotypes, of which some shared a high sequence identity (≥97%) to strain TG39 and the Gulf spill isolates. Other bacterial groups comprising members with well-characterized EPS-producing qualities, such as Alteromonas , Colwellia and Pseudoalteromonas , were also found enriched in surface waters, suggesting that the total pool of EPS in the Gulf during the spill may have been supplemented by these organisms. Roller bottle incubations with one of the Halomonas isolates from the Deepwater Horizon spill site demonstrated its ability to effectively produce oil aggregates and emulsify the oil. The enrichment of EPS-producing bacteria during the spill coupled with their capacity to produce amphiphilic EPS is likely to have contributed to the ultimate removal of the oil and to the formation of oil aggregates, which were a dominant feature observed in contaminated surface waters.
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影响因子：
56.9
作者：
Azam, F
通讯作者：
Azam, F
DOI：
10.1007/0-387-30746-x_28
发表时间：
2006-01-01
期刊：
PROKARYOTES: A HANDBOOK ON THE BIOLOGY OF BACTERIA, VOL 6, THIRD EDITION
影响因子：
--
作者：
Arahal, David R.;Ventosa, Antonio
通讯作者：
Ventosa, Antonio
DOI：
10.1016/0141-8130(95)01038-6
发表时间：
1996-02-01
影响因子：
8.2
作者：
Bozzi, L;Milas, M;Rinaudo, M
通讯作者：
Rinaudo, M
影响因子：
2.7
作者：
BERTRAND, JC;RAMBELOARISOA, E;MATTEI, G
通讯作者：
MATTEI, G
影响因子：
2.6
作者：
Antonio Mata, Juan;Bejar, Victoria;Quesada, Emilia
通讯作者：
Quesada, Emilia