Infrared light excites cells by changing their electrical capacitance.
Infrared light excites cells by changing their electrical capacitance.
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DOI：
10.1038/ncomms1742
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发表时间：
2012-03-13
影响因子：
16.6
通讯作者：
中科院分区：
文献类型：
--
作者：
光学刺激使大脑功能和其他生物过程的研究取得了重要进展，并为从听力恢复到心脏起搏的医疗应用带来了希望。特别是，使用红外波长>1.5 μm的脉冲激光刺激具有治疗潜力，因为它能够直接刺激神经和肌肉，而无需任何遗传或化学预处理。然而，红外刺激的机制一直是一个谜，阻碍了其走向临床。在这里，我们表明，红外光激发细胞通过一种新颖的，高度普遍的静电机制。红外线脉冲被水吸收，使局部温度迅速升高。这种加热可逆地改变质膜的电容，使靶细胞去极化。这种机制是完全可逆的，只需要细胞膜的最基本性质。我们的研究结果强调了脉冲红外刺激的普遍性及其医疗潜力。脉冲红外激光可以直接刺激神经和肌肉，但潜在的生物物理机制仍然是个谜。这项研究表明，红外脉冲通过可逆地改变质膜的电容来去除靶细胞。
Optical stimulation has enabled important advances in the study of brain function and other biological processes, and holds promise for medical applications ranging from hearing restoration to cardiac pace making. In particular, pulsed laser stimulation using infrared wavelengths >1.5 μm has therapeutic potential based on its ability to directly stimulate nerves and muscles without any genetic or chemical pre-treatment. However, the mechanism of infrared stimulation has been a mystery, hindering its path to the clinic. Here we show that infrared light excites cells through a novel, highly general electrostatic mechanism. Infrared pulses are absorbed by water, producing a rapid local increase in temperature. This heating reversibly alters the electrical capacitance of the plasma membrane, depolarizing the target cell. This mechanism is fully reversible and requires only the most basic properties of cell membranes. Our findings underscore the generality of pulsed infrared stimulation and its medical potential. Pulsed infrared laser light can directly stimulate nerves and muscles, but the underlying biophysical mechanism has remained enigmatic. This study reveals that infrared pulses depolarize target cells by reversibly altering the electrical capacitance of the plasma membrane.
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DOI：
10.1523/jneurosci.4190-10.2010
发表时间：
2010-11-10
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Knöpfel T;Lin MZ;Levskaya A;Tian L;Lin JY;Boyden ES
通讯作者：
Boyden ES
影响因子：
13.9
作者：
Fenno L;Yizhar O;Deisseroth K
通讯作者：
Deisseroth K
影响因子：
3.3
作者：
Henderson, Douglas;Boda, Dezso
通讯作者：
Boda, Dezso
影响因子：
3.4
作者：
Wells, Jonathon;Kao, Chris;Jansen, E. Duco
通讯作者：
Jansen, E. Duco
影响因子：
4.5
作者：
PUSCH, M;NEHER, E
通讯作者：
NEHER, E