Identification of fungal enzymes involving 3-phenoxybenzoic acid degradation by using enzymes inhibitors and inducers
Identification of fungal enzymes involving 3-phenoxybenzoic acid degradation by using enzymes inhibitors and inducers
复制标题
使用酶抑制剂和诱导剂鉴定涉及 3-苯氧基苯甲酸降解的真菌酶
DOI：
10.1016/j.mex.2019.100772
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发表时间：
2019-12
期刊：
影响因子：
1.9
通讯作者：
Kai Yao
中科院分区：
文献类型：
--
作者：
Jiayuan Zhao;Xiaofeng Chen;Dongying Jia;Kai Yao
拟除虫菊酯在食品和环境中的残留可生物转化为3-苯氧基苯甲酸（3-PBA），其毒性比母体化合物更强，已在牛奶、土壤和人尿液中检测到。在本研究中，当在30 °C和180 rpm下孵育48 h时，获得对数生长期的菌丝球，并用磷酸盐缓冲液洗涤2次。将诱导和非诱导样品的细胞碎片溶液和滤液与3-PBA及其中间代谢产物在相同条件下培养，通过降解分析酶的定位和诱导。由于真菌中存在的细胞色素P450（CYP 450）、木质素过氧化物酶（LiP）、漆酶、锰过氧化物酶（MNP）和双加氧酶能降解与这些化合物结构相似的污染物，因此选择这些氧化酶作为候选酶，并使用CuSO 4、NaN 3、AgNO 3、EDTA和胡椒基丁醚（PBO）作为酶的抑制剂和诱导剂。通过对3-PBA及其中间代谢产物和真菌生物量在酶抑制剂和诱导剂存在下的降解实验，分析了可能的降解酶，并对共代谢酶和途径进行了推理。该研究为真菌降解3-PBA的共代谢酶的研究提供了一种新的方法。所提出的方法X针对3-PBA降解的共代谢酶和途径进行。·研究了呈现酶特性的培养条件。从酶的定位、诱导作用、真菌酶系统和化合物的化学结构等方面对候选酶进行了分析。
Pyrethroid residues in food and the environment can be bio-transformed into 3-phenoxybenzoic acid (3-PBA); It is more toxic than the parent compounds, and has been detected in milk, soil, and human urine. In this study, when incubated at 30 °C and 180 rpm for 48 h, mycelial pellets during logarithmic growth phase were obtained and washed 2 times by phosphate buffer. The cell debris solutions and filter liquor from inducible and non-inducible samples were cultured with 3-PBA and its intermediate metabolites at same condition, and the location and induction of enzymes were analyzed by the degradation. Then Cytochrome P450 (CYP450), lignin peroxidase (LiP), laccase, manganese peroxidase (MnP), and dioxygenase were selected as candidate enzymes due to these oxidases existing in the fungi and capable of degrading the contaminants with similar structures of these compounds, and CuSO4, NaN3, AgNO3, EDTA or piperonyl butoxide (PBO) were used as the enzymes inhibitors and inducers. The degradation of 3-PBA and its intermediate metabolites and the fungal biomass in presence of enzymes inhibitors and inducers was arranged to analyze the possible degrading-enzymes, and the co-metabolic enzymes and pathways can be reasoned. This study provided a promising method for studying the co-metabolic enzymes of 3-PBA degradation by fungi.•The presented MethodsX was conducted for co-metabolic enzymes and pathways of 3-PBA degradation.•The culturing condition for presenting enzyme properties were investigated.•The candidate enzymes were analyzing based on location, induction of enzymes, fungal enzyme systems and chemical structures of these compounds.
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影响因子：
6.8
作者：
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影响因子：
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作者：
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