Oligodendroglial lineage cells express neuroligand receptors
Oligodendroglial lineage cells express neuroligand receptors
复制标题
少突胶质细胞表达神经配体受体
作者：
C. Kastritsis;K. McCarthy;D. McCarthy
本研究旨在确定少突胶质细胞谱系是否表达与Ca2+动员相关的神经配体受体。用基于视频的成像系统监测细胞内Ca2+水平，并用免疫细胞化学标志物对细胞进行表征。O‐2A祖细胞（A2B5+/GFAP‐）和成熟少突胶质细胞（GC+/MBP+）对去甲肾上腺素、谷氨酸、ATP和组胺有反应，细胞内Ca2+水平升高。当O‐2A祖细胞分化为成熟的少突胶质细胞时，随着细胞内Ca2+水平的增加，对ATP和组胺有反应的细胞百分比增加。O - 2A祖细胞和成熟少突胶质细胞对细胞内Ca2+增加的神经配体的反应能力在药理学上是不同的。缓激肽、碳醇和P物质治疗也增加了O - 2A祖细胞和成熟少突胶质细胞内Ca2+水平。随着O‐2A祖细胞分化为成熟的少突胶质细胞，对缓激肽和P物质有反应的细胞百分比增加，而对碳乙醇有反应的细胞百分比则相反。这些结果表明，少突胶质细胞谱系表现出与Ca2+动员相关的神经配体受体，这些细胞对神经配体的反应能力受到发育调节。©1993 Wiley‐Liss, Inc。
This study was designed to determine whether cells of the oligodendroglial lineage express neuroligand receptors linked to Ca2+ mobilization. Intracellular Ca2+ levels were monitored with a video‐based imaging system and cells were characterized with immunocytochemical markers. O‐2A progenitor cells (A2B5+/GFAP‐) and mature oligodendroglia (GC+/MBP+) responded to norepinephrine, glutamate, ATP, and histamine with increased intracellular Ca2+ levels. As O‐2A progenitor cells differentiated into mature oligodendroglia, there was an increase in the percentage of cells that responded to ATP and histamine with an increase in intracellular Ca2+ levels. Both O‐2A progenitor cells and mature oligodendroglia were pharmacologically heterogeneous with respect to their ability to respond to neuroligands with an increase in intracellular Ca2+. Treatment with bradykinin, carbachol, and substance P also increased intracellular Ca2+ levels in O‐2A progenitor cells and mature oligodendroglia. Whereas the percentage of cells that responded to bradykinin and substance P increased with differentiation of O‐2A progenitor cells into mature oligodendroglia, the trend was reversed with respect to the percentage of cells responding to carbachol. These results suggest that cells of the oligodendroglial lineage exhibit neuroligand receptors linked to Ca2+ mobilization and that the ability of these cells to respond to neuroligands is developmentally regulated. © 1993 Wiley‐Liss, Inc.
少突胶质细胞中 K 电流的表达和调节：在髓鞘形成中的可能作用。
影响因子：
2.9
作者：
Soliven,B;Szuchet,S;Arnason,BG;Nelson,DJ
通讯作者：
Nelson,DJ
影响因子：
4
作者：
ROE, MW;LEMASTERS, JJ;HERMAN, B
通讯作者：
HERMAN, B
环 AMP 调节少突胶质细胞的分化率，而不改变其祖细胞的谱系承诺。
影响因子：
2.7
作者：
Raible,DW;McMorris,FA
通讯作者：
McMorris,FA