Activation of humanα1-antitrypsin gene in rat hepatoma × human fetal liver cell hybrids depends on presence of human chromosome 14
Activation of humanα1-antitrypsin gene in rat hepatoma × human fetal liver cell hybrids depends on presence of human chromosome 14
复制标题
大鼠肝癌×人胎肝细胞杂交体中人α1-抗胰蛋白酶基因的激活取决于人14号染色体的存在
DOI：
--
复制
发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Uta Francke
中科院分区：
文献类型：
--
作者：
S. Pearson;P. Tetri;Donna L. George;Uta Francke
为了研究人类染色体在肝脏特异功能表达中的作用，我们用大鼠肝癌(7777)细胞系与人二倍体皮肤成纤维细胞(XIX系列)或人胎肝细胞(XXII系列)进行了体细胞杂交。通过对浓缩的无血清杂交培养上清液的免疫电泳法检测人血清蛋白的产生。人α-1抗胰蛋白酶(AAT)由杂交瘤细胞亚群分泌，但不能由亲本细胞分泌。激活的人AAT表型在18个初步选择的帽子选择和5个氮鸟嘌呤回选系列XXII中与人类14号染色体分离一致。排除了与AAT表达有关的所有其他染色体。因此，AAT基因PI被分配到14号染色体上。在所研究的其他血清蛋白中没有观察到这种肝脏特异性功能的准结构性表达，也没有在皮肤成纤维细胞来源的杂交体(XIX系列)中观察到这种准构成性表达，尽管其中一些杂交体产生了AAT。
In order to study the involvement of human chromosomes in the expression of liver-specific functions, we have produced somatic cell hybrids between a rat hepatoma (7777) cell line and human diploid skin fibroblasts (series XIX) or human fetal liver cells (series XXII). Production of human serum proteins was detected by immunoelectrophoretic analyses of concentrated serum-free hybrid culture supernatants. Human α1-antitrypsin (AAT) was secreted by a subset of hybrids but not by the parental cells. The activated human AAT phenotype segregated concordantly with human chromosome 14 in 18 primarily HAT-selected and five azaguanine back-selected series XXII hybrids. All other chromosomes were excluded as playing a role in AAT expression. Therefore, the AAT gene PI is assigned to chromosome 14. This quasi-constitutive expression of a liver-specific function was not observed for the other serum proteins studied, nor was it seen in the skin fibroblast-derived hybrids (series XIX) although AAT was produced by some of them.
影响因子：
9.8
作者：
B. de Martinville;A. Wyman;R. White;U. Francke
通讯作者：
B. de Martinville;A. Wyman;R. White;U. Francke