Generation and assessment of high-quality mouse oocytes and embryos following nicotinamide mononucleotide administration.
Generation and assessment of high-quality mouse oocytes and embryos following nicotinamide mononucleotide administration.
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烟酰胺单核苷酸给药后高质量小鼠卵母细胞和胚胎的生成和评估
DOI：
10.1016/j.xpro.2021.100298
复制
发表时间：
2021-03-19
期刊：
影响因子：
--
通讯作者：
Xiong B
中科院分区：
文献类型：
--
作者：
Miao Y;Chen J;Gao Q;Xiong B
卵母细胞的低质量是高龄雌性哺乳动物生殖结果不理想的主要原因之一。在这里，我们提出了一个详细的协议，以获得高质量的卵母细胞和胚胎从老年小鼠的烟酰胺单核苷酸（NMN）管理。我们还描述了荧光染色程序来评估卵母细胞中的细胞器动态，并在体外受精和胚胎培养系统中评估NMN对受精能力和胚胎发育潜力的影响。有关本方案使用和执行的完整信息，请参见。通过NMN处理收获高质量小鼠卵母细胞和胚胎的方案荧光染色和共聚焦成像以评估卵母细胞质量体外受精和胚胎培养程序以评估胚胎质量卵母细胞质量低是高龄雌性哺乳动物生殖结果不佳的主要原因之一。在这里，我们提出了一个详细的协议，以获得高质量的卵母细胞和胚胎从老年小鼠的烟酰胺单核苷酸（NMN）管理。我们还描述了荧光染色程序来评估卵母细胞中的细胞器动态，并在体外受精和胚胎培养系统中评估NMN对受精能力和胚胎发育潜力的影响。
The low quality of oocytes is one of the main causes of the suboptimal reproductive outcome of female mammals with advanced maternal age. Here, we present a detailed protocol to obtain high-quality oocytes and embryos from aged mice by nicotinamide mononucleotide (NMN) administration. We also describe fluorescence staining procedures to assess the organelle dynamics in oocytes, and in vitro fertilization and embryo culture systems to evaluate the influence of NMN on the fertilization ability and embryonic development potential. For complete information on the use and execution of this protocol, please refer to. A protocol for harvest of high-quality mouse oocytes and embryos by NMN treatment Fluorescence staining and confocal imaging to evaluate oocyte quality In vitro fertilization and embryo culture procedures to assess embryo quality The low quality of oocytes is one of the main causes of the suboptimal reproductive outcome of female mammals with advanced maternal age. Here, we present a detailed protocol to obtain high-quality oocytes and embryos from aged mice by nicotinamide mononucleotide (NMN) administration. We also describe fluorescence staining procedures to assess the organelle dynamics in oocytes, and in vitro fertilization and embryo culture systems to evaluate the influence of NMN on the fertilization ability and embryonic development potential.
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DOI：
10.1111/j.1749-6632.2010.05521.x
发表时间：
2010-01-01
期刊：
REPRODUCTIVE AGING
影响因子：
--
作者：
Traub, Michael L.;Santoro, Nanette
通讯作者：
Santoro, Nanette
排卵后衰老通过诱导氧化应激导致猪卵母细胞恶化。
影响因子：
4.8
作者：
Miao, Yilong;Zhou, Changyin;Xiong, Bo
通讯作者：
Xiong, Bo
影响因子：
29
作者：
Yoshino J;Baur JA;Imai SI
通讯作者：
Imai SI
影响因子：
7.8
作者：
de Picciotto NE;Gano LB;Johnson LC;Martens CR;Sindler AL;Mills KF;Imai S;Seals DR
通讯作者：
Seals DR
影响因子：
64.5
作者：
Gomes AP;Price NL;Ling AJ;Moslehi JJ;Montgomery MK;Rajman L;White JP;Teodoro JS;Wrann CD;Hubbard BP;Mercken EM;Palmeira CM;de Cabo R;Rolo AP;Turner N;Bell EL;Sinclair DA
通讯作者：
Sinclair DA