Complex cytokeratin polypeptide patterns observed in certain human carcinomas.
Complex cytokeratin polypeptide patterns observed in certain human carcinomas.
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在某些人类癌症中观察到复杂的细胞角蛋白多肽模式。
DOI：
10.1111/j.1432-0436.1982.tb01291.x
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
W. Franke
中科院分区：
文献类型：
--
作者：
R. Moll;R. Krepler;W. Franke
人上皮细胞含有中等大小的细丝，由与表皮α-角蛋白(“细胞角蛋白”)相关的多肽组成，在不同的上皮细胞中以不同的组合表达。我们利用人的活检和尸检的细胞骨架蛋白，通过双向凝胶电泳和免疫印迹实验，检测了不同原发和转移癌的细胞角蛋白多肽图谱，并将其与相应的正常上皮组织和培养细胞进行了比较。可以区分五组癌细胞角蛋白的模式。(1)典型的单纯性上皮细胞角蛋白(多肽7、8、18、19)以不同的组合在许多腺癌中表达，例如胃肠道腺癌。(2)在皮肤和舌鳞状细胞癌中可见典型的复层上皮细胞角蛋白(编号1、5、6、10、11、14-17)。(3)在某些呼吸道和乳腺癌中检测到显示7、8、18、19号多肽和一个基本成分(5号或6号)的复杂图案。(4)含有广泛存在于复层上皮中的细胞角蛋白(4-6、14-17)以及8号和19号成分的复杂图案存在于来自非角化复层上皮的各种鳞癌中，并伴有或不带有少量额外的18号细胞角蛋白。(5)在一些罕见的肿瘤中可以发现异常高的复杂性图案，如泄殖腔原癌所示。比较原发肿瘤和转移瘤时，细胞角蛋白的表达没有明显的质变。与正常上皮细胞角蛋白模式相比，第一类癌通常表现出与起源组织高度相关。其他癌不表达其起源组织中存在的某些细胞角蛋白，反之亦然，某些在正常组织中不存在或明显缺失的成分是相应肿瘤中的主要细胞角蛋白。这些差异可以通过癌变过程中的细胞类型选择来解释，但不能完全排除在肿瘤发生过程中表达的变化。还讨论了特定肿瘤内细胞类型异质性的可能性。在某些培养的人类癌细胞系(例如，A-431、RPMI2650、Detroit 562、A-549)中也发现了类似的复杂的细胞角蛋白多肽模式，也可以在细胞克隆中观察到。本文还讨论了用凝胶电泳法或特异性单抗分析细胞角蛋白图谱，以非形态标准区分不同的癌的可能价值。
Human epithelial cells contain, intermediate-sized filaments formed by polypeptides related to epidermal alpha-keratin ("cytokeratins") which are expressed in different combinations in different epithelia. Using cytoskeletal proteins from human biopsies and autopsies we have examined, by two-dimensional gel electrophoresis and immunoblotting experiments, the cytokeratin polypeptide patterns of diverse primary and metastatic carcinomas and have compared them with those of corresponding normal epithelial tissues and cultured cells. Five groups of carcinoma cytokeratin patterns can be discriminated. (1) Cytokeratins typical of simple epithelia (polypeptides Nos. 7, 8, 18, 19) are expressed, in various combinations, by many adenocarcinomas, for example those of gastrointestinal tract. (2) Cytokeratins typical of stratified epithelia (Nos. 1, 5, 6, 10, 11, 14-17) are found, in various combinations, in squamous cell carcinomas of skin and tongue. (3) Complex patterns showing polypeptides Nos. 7, 8, 18, 19, and one basic component (No. 5 or 6) are detected in certain carcinomas of the respiratory tract and the breast. (4) Complex patterns containing cytokeratins widespread in stratified epithelia (Nos. 4-6, 14-17) as well as components Nos. 8 and 19 occur in diverse squamous cell carcinomas derived from non-cornified stratified epithelia, with or without additional small amounts of cytokeratin No. 18. (5) Patterns of unusually high complexity can be found in some rare tumors as is shown for a cloacogenic carcinoma. No significant qualitative changes of expression of cytokeratins were found when primary tumors and metastases were compared. When compared with cytokeratin patterns of normal epithelia, carcinomas of the first type usually display a high degree of relatedness to the tissue of origin. Other carcinomas do not express some of the cytokeratins present in the tissue of their origin and, vice versa, certain components which are minor or apparently absent in normal tissue are major cytokeratins in the corresponding tumor. These differences may be explained by cell type selection during carcinogenesis, but changes of expression during tumor development cannot be categorically excluded. The possibility of cell type heterogeneity within a given tumor is also discussed. Similarly complex patterns of cytokeratin polypeptides have been noted in certain cultured human carcinoma cell lines (e.g., A-431, RPMI 2650, Detroit 562, A-549) and can also be observed in cell clones. The possible value of analyses of cytokeratin patterns, by gel electrophoresis or specific monoclonal antibodies, in distinguishing different carcinomas by non-morphologic criteria is discussed.
使用抗角蛋白抗血清作为诊断工具：胸腺瘤与淋巴瘤。
影响因子：
3.3
作者：
Battifora,H;Sun,TT;Bahu,RM;Rao,S
通讯作者：
Rao,S
Novikoff 腹水肝癌细胞角蛋白抗原的鉴定。
DOI：
10.1073/pnas.79.10.3138
发表时间：
1982
影响因子：
11.1
作者：
Schmidt,WN;Pardue,RL;Tutt,MC;Briggs,RC;Brinkley,BR;Hnilica,LS
通讯作者：
Hnilica,LS