Fecal carriage of multidrug-resistant Escherichia coli by community children in southern Taiwan.
Fecal carriage of multidrug-resistant Escherichia coli by community children in southern Taiwan.
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DOI：
10.1186/s12876-018-0807-x
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发表时间：
2018-06-15
影响因子：
2.4
通讯作者：
Cheng MF
中科院分区：
文献类型：
--
作者：
Huang IF;Lee WY;Wang JL;Hung CH;Hu HH;Hung WY;Hung YJ;Chen WC;Shen YT;Cheng MF
多药耐药大肠埃希菌（Escherichia coli，E. coli），特别是E. coli序列型ST 131，正成为全球关注的问题。共生细菌是抗生素耐药基因的重要储存库，促进了这些基因向病原细菌菌株的传播。本研究的目的是调查粪便携带MDR E。coli和ST 131 E.台湾南部社区儿童大肠杆菌感染率。在这项前瞻性研究中，在2013年10月至2014年9月住院3天内采集了0-18岁儿童的粪便样本。排除了在标本采集前3个月内有基础疾病、抗生素治疗或住院史的儿童。E.大肠埃希菌菌落进行药敏试验、O25 b-ST 131、多位点序列分型和blaCTX-M基因群检测。在157株E.对氨苄西林、阿莫西林+克拉维酸、复方新诺明和头孢唑啉的不敏感率分别为70%、65.6%、47.1%和32.5%。29株（18.5%）对环丙沙星不敏感。MDR大肠大肠埃希菌占58株（37%）。13株（8.3%）产超广谱β-内酰胺酶（ESBL）。O25 b阳性26株（16.6%），ST 131阳性13株（8.3%）。13株产ESBL的E.大肠埃希菌属于blaCTX-M群9株，其中CTXM-14株，O25 b-ST 131阳性4株（80%）。与非ESBL和环丙沙星敏感组相比，ESBL和环丙沙星非敏感组O25 b-ST 131阳性率显著增高。非敏感E.大肠埃希菌在儿童中的检出率较高;大肠埃希菌耐药率为37%，环丙沙星不敏感率为18.5%，产ESBL率为8.3%。O25 b-ST 131是最常见的产ESBL大肠杆菌。大肠埃希菌克隆群在儿童粪便中检出率最高，ESBL和环丙沙星非敏感组O25 b-ST 131阳性率明显高于非敏感组。本文的在线版本（10.1186/s12876-018-0807-x）包含补充材料，可供授权用户使用。
The emergence of multidrug-resistant (MDR) Escherichia coli (E. coli), particularly E. coli sequence type ST131, is becoming a global concern. Commensal bacteria, an important reservoir of antibiotic resistance genes, facilitate the spread of such genes to pathogenic bacterial strains. The objective of the study is to investigate the fecal carriage of MDR E. coli and ST131 E. coli in community children in Southern Taiwan. In this prospective study, stool samples from children aged 0–18 years were obtained within 3 days of hospitalization from October 2013 to September 2014. Children with a history of underlying diseases, antibiotic treatment, or hospitalization in the 3 months before specimen collection were excluded. E. coli colonies were selected and tested for antimicrobial susceptibility, and O25b-ST131, multilocus sequence typing, and blaCTX-M gene groups were detected. Among 157 E. coli isolates, the rates of nonsusceptibility to ampicillin, amoxycillin + clavulanate, trimethoprim–sulfamethoxazole, and cefazolin were 70, 65.6, 47.1, and 32.5%, respectively. Twenty-nine (18.5%) isolates were nonsusceptible to ciprofloxacin. MDR E. coli accounted for 58 (37%) of all isolates. Thirteen (8.3%) isolates produced extended-spectrum β-lactamase (ESBL). Furthermore, 26 (16.6%) and 13 (8.3%) isolates were O25b and ST131 positive, respectively. Five (38.5%) of the 13 ESBL-producing E. coli belonged to blaCTX-M group 9, among which were CTXM-14 and 4 (80%) were O25b–ST131 positive. Compared with the non-ESBL and ciprofloxacin-susceptible groups, the ESBL and ciprofloxacin-nonsusceptible groups showed significantly higher rates of O25b–ST131 positivity. The prevalence of the fecal carriage of nonsusceptible E. coli in children was high; among these E. coli, 37% were MDR, 18.5% were nonsusceptible to ciprofloxacin, and 8.3% produced ESBL. O25b–ST131 was the most common ESBL-producing E. coli clonal group present in the feces of children, and the ESBL and ciprofloxacin-nonsusceptible groups showed significantly higher rates of O25b–ST131 positivity. The online version of this article (10.1186/s12876-018-0807-x) contains supplementary material, which is available to authorized users.
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影响因子：
2.6
作者：
Guimaraes, Bruno;Barreto, Angela;Poeta, Patricia
通讯作者：
Poeta, Patricia
影响因子：
14.2
作者：
Magiorakos, A. -P.;Srinivasan, A.;Monnet, D. L.
通讯作者：
Monnet, D. L.
DOI：
10.1186/1476-0711-13-22
发表时间：
2014-06-16
影响因子：
5.7
作者：
Ahmed SF;Ali MM;Mohamed ZK;Moussa TA;Klena JD
通讯作者：
Klena JD
影响因子：
3.7
作者：
Isendahl J;Turlej-Rogacka A;Manjuba C;Rodrigues A;Giske CG;Nauclér P
通讯作者：
Nauclér P
影响因子：
5.2
作者：
Dallenne, Caroline;Da Costa, Anaelle;Arlet, Guillaume
通讯作者：
Arlet, Guillaume