Physiology of a Hybrid Pathway for Nicotine Catabolism in Bacteria.
Physiology of a Hybrid Pathway for Nicotine Catabolism in Bacteria.
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DOI：
10.3389/fmicb.2020.598207
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发表时间：
2020
影响因子：
5.2
通讯作者：
Wang S
中科院分区：
文献类型：
--
作者：
Huang H;Shang J;Wang S
尼古丁是烟草中主要的氮杂环类生物碱，也是烟草废弃物中的主要有毒化学物质。由于其复杂的生理效应和毒性，它已成为公众健康和环境方面的一个问题。属于节杆菌属和假单胞菌属的许多细菌可以通过吡啶和吡咯烷途径降解尼古丁。最近，在根瘤菌群细菌Agrobacterium tumefaciens S33、Shinella sp. HZN7和Ochrobactrum sp. SJY 1以及其他群细菌中发现了一种新的吡啶和吡咯烷途径（也称为VPP途径）的杂合。这一特殊的镶嵌途径引起了微生物学家的广泛关注，并对其分子和生化机制进行了深入研究。这将有利于开发新的生物技术，包括尼古丁的使用、参与尼古丁分解的酶以及能够降解生物碱的微生物。在这一途径中，一些代谢产物在吡啶环上被羟基化或在侧链上被活性基团修饰，可作为前体用于合成制药和农业工业中的一些重要化合物。此外，一些酶可用于工业生物催化，以将吡啶衍生物转化为所需的化学品。在这里，我们回顾了混合尼古丁降解途径的分子和生物化学基础，并讨论了其氧化降解的电子传递能量守恒和细菌生长。
Nicotine is a major N-heterocyclic aromatic alkaloid produced in tobacco plants and the main toxic chemical in tobacco waste. Due to its complex physiological effects and toxicity, it has become a concern both in terms of public health and the environment. A number of bacteria belonging to the genera Arthrobacter and Pseudomonas can degrade nicotine via the pyridine and pyrrollidine pathways. Recently, a novel hybrid of the pyridine and pyrrolidine pathways (also known as the VPP pathway) was found in the Rhizobiale group bacteria Agrobacterium tumefaciens S33, Shinella sp. HZN7 and Ochrobactrum sp. SJY1 as well as in other group bacteria. The special mosaic pathway has attracted much attention from microbiologists in terms of the study of their molecular and biochemical mechanisms. This will benefit the development of new biotechnologies in terms of the use of nicotine, the enzymes involved in its catabolism, and the microorganisms capable of degrading the alkaloid. In this pathway, some metabolites are hydroxylated in the pyridine ring or modified in the side chain with active groups, which can be used as precursors for the synthesis of some important compounds in the pharmaceutical and agricultural industries. Moreover, some enzymes may be used for industrial biocatalysis to transform pyridine derivatives into desired chemicals. Here, we review the molecular and biochemical basis of the hybrid nicotine-degrading pathway and discuss the electron transport in its oxidative degradation for energy conservation and bacterial growth.
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影响因子：
4.6
作者：
Huang H;Yu W;Wang R;Li H;Xie H;Wang S
通讯作者：
Wang S
系统地揭示假单胞菌中尼古丁降解的未解途径
影响因子：
4.5
作者：
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4.4
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影响因子：
4.4
作者：
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通讯作者：
Liu, Weiping
影响因子：
3.7
作者：
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通讯作者：
Wang S