Genomic and transcriptomic analyses of Agrobacterium tumefaciens S33 reveal the molecular mechanism of a novel hybrid nicotine-degrading pathway.
Genomic and transcriptomic analyses of Agrobacterium tumefaciens S33 reveal the molecular mechanism of a novel hybrid nicotine-degrading pathway.
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DOI：
10.1038/s41598-017-05320-1
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发表时间：
2017-07-06
影响因子：
4.6
通讯作者：
Wang S
中科院分区：
文献类型：
--
作者：
Huang H;Yu W;Wang R;Li H;Xie H;Wang S
农杆菌农杆菌S33能够通过吡啶和吡咯烷的新型杂交途径降解尼古丁。它可以从烟草废弃物中脱除尼古丁，并将尼古丁转化为重要的功能化吡啶前体，用于某些有价药物和杀虫剂。然而，杂交途径的分子机制尚不完全清楚。本文报道了菌株S33在葡萄糖-铵培养基和尼古丁培养基中生长的基因组分析及其转录组。尼古丁分解代谢的完整基因簇位于一个基因组岛上，该基因组岛由与吡啶和吡罗烷途径功能相似但序列不同的基因，以及编码质粒分配和复制起始蛋白、共轭转移蛋白和转座酶的基因组成。这表明，这种杂交途径的进化不是两种途径中涉及的基因的简单融合，而是一个复杂的横向基因转移的结果。此外，其他可能参与杂交途径的基因可能包括尼古丁降解过程中负责底物感知和运输、转录调控和电子转移的基因。该研究为尼古丁降解新途径的分子机制提供了新的见解。
Agrobacterium tumefaciens S33 is able to degrade nicotine via a novel hybrid of the pyridine and pyrrolidine pathways. It can be utilized to remove nicotine from tobacco wastes and transform nicotine into important functionalized pyridine precursors for some valuable drugs and insecticides. However, the molecular mechanism of the hybrid pathway is still not completely clear. Here we report the genome analysis of strain S33 and its transcriptomes grown in glucose-ammonium medium and nicotine medium. The complete gene cluster involved in nicotine catabolism was found to be located on a genomic island composed of genes functionally similar but not in sequences to those of the pyridine and pyrrolidine pathways, as well as genes encoding plasmid partitioning and replication initiation proteins, conjugal transfer proteins and transposases. This suggests that the evolution of this hybrid pathway is not a simple fusion of the genes involved in the two pathways, but the result of a complicated lateral gene transfer. In addition, other genes potentially involved in the hybrid pathway could include those responsible for substrate sensing and transport, transcription regulation and electron transfer during nicotine degradation. This study provides new insights into the molecular mechanism of the novel hybrid pathway for nicotine degradation.
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