COVID-19-Associated Pulmonary Aspergillosis Isolates Are Genomically Diverse but Similar to Each Other in Their Responses to Infection-Relevant Stresses.
COVID-19-Associated Pulmonary Aspergillosis Isolates Are Genomically Diverse but Similar to Each Other in Their Responses to Infection-Relevant Stresses.
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DOI：
10.1128/spectrum.05128-22
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发表时间：
2023-03-22
影响因子：
3.7
通讯作者：
ECMM CAPA Study Grp
中科院分区：
文献类型：
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作者：
Mead, Matthew E.;de Castro, Patricia Alves L.;Steenwyk, Jacob L.;Gangneux, Jean-Pierre;Hoenigl, Martin;Prattes, Juergen;Rautemaa-Richardson, Riina;Guegan, Helene B.;Moore, Caroline B.;Lass-Floerl, Cornelia;Reizine, Florian;Valero, Clara;Van Rhijn, Norman J.;Bromley, Michael J.;Rokas, Antonis H.;Goldman, Gustavo H.;Gago, Sara;ECMM CAPA Study Grp
由肺部真菌病原体烟曲霉引起的继发感染是严重冠状病毒病19（COVID-19）患者死亡的重要原因。尽管上皮细胞损伤和异常细胞因子反应与COVID-19相关肺曲霉病（CAPA）的易感性有关，但对致病性的基础机制知之甚少。在此，我们分析了11 A的基因组。来自不同欧洲国家的三个中心CAPA患者的烟曲霉分离株。CAPA分离株在代表性的A.烟曲霉菌株在表型上，CAPA分离株与A.在与感染相关的应激中生长时，烟曲霉A1160参考菌株与Af 293菌株相比具有显著的差异，除了与人免疫细胞的相互作用，其中巨噬细胞应答与Af 293参考菌株诱导的那些相似。总的来说，我们的数据表明CAPA分离株基因组多样，但在对感染相关压力的反应方面彼此更相似。应研究来自CAPA患者的大量分离株，以更好地了解CAPA的分子流行病学，并确定COVID-19患者中共致病性和抗真菌药耐药性的遗传驱动因素。2019冠状病毒病（COVID-19）相关肺曲霉病（CAPA）已被全球报告为部分严重COVID-19患者的危及生命的并发症。这些感染大多数是由环境霉菌烟曲霉引起的，烟曲霉在世界卫生组织真菌病原体优先名单中排名第三。然而，很少有人知道烟曲霉CAPA菌株的分子流行病学。在此，我们分析了11 A的基因组。在来自不同欧洲国家的三个中心，从患有CAPA的患者分离烟曲霉菌株，并进行表型分析，以了解疾病的病理生理学。我们的数据表明A.烟曲霉CAPA分离物在基因组上是多样的，但在它们对感染相关胁迫的反应中彼此更相似。
Secondary infections caused by the pulmonary fungal pathogen Aspergillus fumigatus are a significant cause of mortality in patients with severe coronavirus disease 19 (COVID-19). Even though epithelial cell damage and aberrant cytokine responses have been linked to susceptibility to COVID-19-associated pulmonary aspergillosis (CAPA), little is known about the mechanisms underpinning copathogenicity. Here, we analyzed the genomes of 11 A. fumigatus isolates from patients with CAPA in three centers from different European countries. CAPA isolates did not cluster based on geographic origin in a genome-scale phylogeny of representative A. fumigatus isolates. Phenotypically, CAPA isolates were more similar to the A. fumigatus A1160 reference strain than to the Af293 strain when grown in infection-relevant stresses, except for interactions with human immune cells wherein macrophage responses were similar to those induced by the Af293 reference strain. Collectively, our data indicate that CAPA isolates are genomically diverse but are more similar to each other in their responses to infection-relevant stresses. A larger number of isolates from CAPA patients should be studied to better understand the molecular epidemiology of CAPA and to identify genetic drivers of copathogenicity and antifungal resistance in patients with COVID-19. IMPORTANCE Coronavirus disease 2019 (COVID-19)-associated pulmonary aspergillosis (CAPA) has been globally reported as a life-threatening complication in some patients with severe COVID-19. Most of these infections are caused by the environmental mold Aspergillus fumigatus, which ranks third in the fungal pathogen priority list of the WHO. However, little is known about the molecular epidemiology of Aspergillus fumigatus CAPA strains. Here, we analyzed the genomes of 11 A. fumigatus isolates from patients with CAPA in three centers from different European countries, and carried out phenotypic analyses with a view to understanding the pathophysiology of the disease. Our data indicate that A. fumigatus CAPA isolates are genomically diverse but are more similar to each other in their responses to infection-relevant stresses.
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影响因子：
2.9
作者：
Bertuzzi M;van Rhijn N;Krappmann S;Bowyer P;Bromley MJ;Bignell EM
通讯作者：
Bignell EM
DOI：
10.1093/bioinformatics/8.3.275
发表时间：
1992-06-01
期刊：
COMPUTER APPLICATIONS IN THE BIOSCIENCES
影响因子：
--
作者：
JONES, DT;TAYLOR, WR;THORNTON, JM
通讯作者：
THORNTON, JM
DOI：
10.1016/j.fgb.2018.02.003
发表时间：
2018-04
期刊：
Fungal genetics and biology : FG & B
影响因子：
--
作者：
Ballard E;Melchers WJG;Zoll J;Brown AJP;Verweij PE;Warris A
通讯作者：
Warris A
影响因子：
28.3
作者：
通讯作者：
--
DOI：
10.15252/embj.201489468
发表时间：
2014-10-01
期刊：
The EMBO journal
影响因子：
--
作者：
Gsaller F;Hortschansky P;Beattie SR;Klammer V;Tuppatsch K;Lechner BE;Rietzschel N;Werner ER;Vogan AA;Chung D;Mühlenhoff U;Kato M;Cramer RA;Brakhage AA;Haas H
通讯作者：
Haas H