Dact1-3 mRNAs exhibit distinct expression domains during tooth development.
Dact1-3 mRNAs exhibit distinct expression domains during tooth development.
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DOI：
10.1016/j.gep.2010.02.002
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发表时间：
2010-02
期刊：
影响因子：
--
通讯作者：
Luukko K
中科院分区：
文献类型：
--
作者：
Kettunen P;Kivimäe S;Keshari P;Klein OD;Cheyette BN;Luukko K
Wnt信号对牙齿形成至关重要。Dact蛋白通过与细胞内蛋白Dishevelled（Dvl）结合来调节Wnt信号传导。比较所有已知的小鼠Dact基因，Dact 1 -3，从下颌第一磨牙发育的形态开始后，采用部分原位杂交的根形成的发病表现出不同的，互补的和重叠的表达模式的研究基因。虽然Dact 2的表达仅限于牙上皮细胞，包括釉结信号中心和牙齿特异性成纤维细胞，Dact 1和Dact 3显示发育调控的表达在牙齿间充质。这两种mRNA首先在假定的牙齿间充质中检测到。从芽期牙胚的致密牙间充质下调后，Dact 1在帽期的牙囊间充质中上调，随后在钟期的牙乳头中也上调，其中表达持续到出生后阶段。与此相反，Dact 3转录持续整个牙齿间充质组织成分，包括牙齿特异性细胞，preodontoblasts之前，转录在很大程度上下调从出生后的牙胚。总的来说，这些结果表明，Dact 1和-3可能有助于通过在间充质，包括preodontoblasts的Wnt信号通路的调制早期牙齿形成，而Dact 2可能发挥重要的信号调节作用，在相邻的上皮细胞，包括釉结信号中心和成釉细胞。未来的功能丧失研究将有助于阐明这些功能中是否有多余的功能，特别是Dact 1和Dact 3。
Wnt signaling is essential for tooth formation. Dact proteins modulate Wnt signaling by binding to the intracellular protein Dishevelled (Dvl). Comparison of all known mouse Dact genes, Dact1-3, from the morphological initiation of mandibular first molar development after the onset of the root formation using sectional in situ hybridization showed distinct, complementary and overlapping expression patterns for the studied genes. While Dact2 expression was restricted to the dental epithelium including the enamel knot signaling centers and tooth specific preameloblasts, Dact1 and Dact3 showed developmentally regulated expression in the dental mesenchyme. Both mRNAs were first detected in the presumptive dental mesenchyme. After being downregulated from the condensed dental mesenchyme of the bud stage tooth germ, Dact1 was upregulated in the dental follicle masenchyme at the cap stage and subsequently also in the dental papilla at the bell stage where the expression persisted to the postnatal stages. In contrast, Dact3 transcripts persisted throughout the dental mesenchymal tissue components including the tooth-specific cells, preodontoblasts before transcripts were largely downregulated from the tooth germ postnatally. Collectively these results suggest that Dact1 and -3 may contribute to early tooth formation by modulation of Wnt signaling pathways in the mesenchyme, including preodontoblasts, whereas Dact2 may play important signal-modulating roles in the adjacent epithelial cells including the enamel knot signaling centers and preameloblasts. Future loss-of-function studies will help elucidate whether any of these functions are redundant, particularly for Dact1 and Dact3.
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DOI：
10.1073/pnas.97.26.14444
发表时间：
2000-12-19
影响因子：
11.1
作者：
Jernvall, J;Keränen, SVE;Thesleff, I
通讯作者：
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发表时间：
2006-12-05
影响因子：
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作者：
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