Analysis of cDNA sequences of feline SAAs
Analysis of cDNA sequences of feline SAAs
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猫 SAA 的 cDNA 序列分析
DOI：
10.1080/13506120410001676836
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发表时间：
2004
期刊：
影响因子：
5.5
通讯作者：
E. Gruys
中科院分区：
文献类型：
--
作者：
Maartje M van Rossum;F. V. van Asten;J. Rofina;J. Lenstra;M. Benson;E. Gruys
猫淀粉样变性是一种罕见的疾病，由淀粉样蛋白沉积在多种器官中引起。最常见的类型是老年猫的胰岛淀粉样蛋白 (AIAPP) 衍生的淀粉样蛋白，以及阿比西尼亚/索马里 (Aby) 和暹罗/东方 (Siam) 猫的载脂蛋白、脱脂血清淀粉样蛋白 A (AA) 衍生的淀粉样蛋白，发生在相对年轻的年龄。对于阿比猫、暹罗猫和家养短毛猫（DSH）品种的 AA 蛋白，已经描述了不同的氨基酸序列。然而，目前尚不清楚阿比和暹罗患 AA 淀粉样变的倾向是否与特定的 apoSAA 蛋白序列有关，以及这是否具有品种特异性。在这项研究中，一只暹罗猫和两只 DSH 猫的 DNA 在 Southern blot 上显示出 3 个条带，表明至少有 3 个基因或基因簇。通过 RT-PCR 扩增、克隆和测序来自 3 个 Abys、5 个 Siams 和 5 个 DSH 的肝 mRNA 的 SAA cDNA。暹罗和阿比斯的 SAA 序列变异性有限。所有五只暹罗猫（其中三只呈淀粉样蛋白阳性）均具有淀粉样变性暹罗 SAA 和淀粉样变性阿比 SAA 序列。其中两个 Aby 都含有淀粉样蛋白，具有淀粉样蛋白形成的 Aby SAA 序列。第三个 Aby 没有淀粉样蛋白，错过了其淀粉样蛋白生成序列。在目前的初步调查中发现的 DSH 的 SAA 序列表明可能存在更多变异的趋势，而淀粉样变性 Siam 和淀粉样变性 Aby 序列都曾被发现。在一只动物中发现了多达五个不同的序列。此外，所有五个 DSH 都具有暹罗群岛和阿比群岛所缺乏的特定序列。目前的结果，特别是暹罗人的结果，表明除了淀粉样蛋白相关SAA基因的发生外，其他因素如感染和炎症过程也参与表型淀粉样变性的发展。
Feline amyloidosis is an uncommon disorder caused by deposits of amyloid in a variety of organs. Most frequently encountered types are amyloid derived of pancreatic islet amyloid polypeptide (AIAPP) in older cats and of the apolipoprotein, apo-serum amyloid A (AA) in Abyssinian/Somali (Aby) and Siamese/Oriental (Siam) cats occurring at a relatively young age. For the AA protein of the Aby, Siam and domestic shorthair cat (DSH) breed different amino acid sequences have been described. It is not yet clear, however, whether the tendency to develop AA amyloidosis in Aby and Siam is associated with specific apoSAA protein sequences and whether this is breed specific. In this study, DNA from one Siam and two DSH cats revealed on Southern blot three bands suggesting at least three genes or gene clusters. The SAA cDNAs of hepatic mRNA from three Abys, five Siams and five DSHs were amplified by RT-PCR, cloned and sequenced. Siams and Abys had limited SAA sequence variability. All five Siams, three of which were positive for amyloid, had the amyloidogenic Siam SAA and the amyloidogenic Aby SAA sequence. Two of the Abys, both with amyloid, had the amyloidogenic Aby SAA sequence. The third Aby, without amyloid, missed its amyloidogenic sequence. The SAA sequences of the DSHs found in the present preliminary survey, suggested a possible tendency for more variability, whereas the amyloidogenic Siam as well as the amyloidogenic Aby sequence were found once. Up to five different sequences were found in a single animal. All five DSHs, moreover, had a specific sequence lacking in the Siams and Abys. The present results, especially those of the Siams, favor that in addition to the occurrence of amyloid associated SAA genes other factors such as infections and inflammatory processes are involved in the development of phenotypical amyloidosis.
阿比西尼亚猫淀粉样蛋白 AA 的分离和表征。
DOI：
--
发表时间：
1985
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
DiBartola,SP;Benson,MD;Dwulet,FE;Cornacoff,JB
通讯作者：
Cornacoff,JB
新血清淀粉样蛋白 A 基因的序列和结构。
影响因子：
14.9
作者：
Stearman,RS;Lowell,CA;Peltzman,CG;Morrow,JF
通讯作者：
Morrow,JF
人血清淀粉样蛋白 A (SAA)：SAA 前体和由互补 DNA 定义的结构变体的生物合成和合成后加工。
影响因子：
2.9
作者：
Sipe,JD;Colten,HR;Goldberger,G;Edge,MD;Tack,BF;Cohen,AS;Whitehead,AS
通讯作者：
Whitehead,AS