Top-Down Chemoenzymatic Approach to Synthesizing Diverse High-Mannose N-Glycans and Related Neoglycoproteins for Carbohydrate Microarray Analysis.
Top-Down Chemoenzymatic Approach to Synthesizing Diverse High-Mannose N-Glycans and Related Neoglycoproteins for Carbohydrate Microarray Analysis.
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DOI：
10.1021/acs.bioconjchem.8b00145
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发表时间：
2018-06-20
影响因子：
4.7
通讯作者：
Wang LX
中科院分区：
文献类型：
--
作者：
Toonstra C;Wu L;Li C;Wang D;Wang LX
High-mannose-type N-glycans are an important component of neutralizing epitopes on HIV-1 envelope glycoprotein gp120. They also serve as signals for protein folding, trafficking, and degradation in protein quality control. A number of lectins and antibodies recognize high-mannose-type N-glycans, and glycan array technology has provided an avenue to probe these oligomannose-specific proteins. We describe in this paper a top-down chemoenzymatic approach to synthesize a library of high-mannose N-glycans and related neoglycoproteins for glycan microarray analysis. The method involves the sequential enzymatic trimming of two readily available natural N-glycans, the Man9GlcNAc2Asn prepared from soybean flour and the sialoglycopeptide (SGP) isolated from chicken egg yolks, coupled with chromatographic separation to obtain a collection of a full range of natural high-mannose N-glycans. The Asn-linked N-glycans were conjugated to bovine serum albumin (BSA) to provide neoglycoproteins containing the oligomannose moieties. The glycoepitopes displayed were characterized using an array of glycan-binding proteins, including the broadly virus-neutralizing agents, glycan-specific antibody 2G12, Galanthus nivalis lectin (GNA), and Narcissus pseudonarcissus lectin (NPA).
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影响因子：
3.7
作者：
Ritchie G;Harvey DJ;Feldmann F;Stroeher U;Feldmann H;Royle L;Dwek RA;Rudd PM
通讯作者：
Rudd PM
影响因子：
2.9
作者：
Song X;Lasanajak Y;Rivera-Marrero C;Luyai A;Willard M;Smith DF;Cummings RD
通讯作者：
Cummings RD
影响因子：
4.4
作者：
Go, Eden P.;Chang, Qing;Liao, Hua-Xin;Sutherland, Laura L.;Alam, S. Munir;Haynes, Barton F.;Desaire, Heather
通讯作者：
Desaire, Heather
影响因子：
4.4
作者：
Go EP;Liao HX;Alam SM;Hua D;Haynes BF;Desaire H
通讯作者：
Desaire H
DOI：
10.1126/science.1213256
发表时间：
2011-11-25
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Pejchal R;Doores KJ;Walker LM;Khayat R;Huang PS;Wang SK;Stanfield RL;Julien JP;Ramos A;Crispin M;Depetris R;Katpally U;Marozsan A;Cupo A;Maloveste S;Liu Y;McBride R;Ito Y;Sanders RW;Ogohara C;Paulson JC;Feizi T;Scanlan CN;Wong CH;Moore JP;Olson WC;Ward AB;Poignard P;Schief WR;Burton DR;Wilson IA
通讯作者：
Wilson IA