Bmi-1 high-expressing cells enrich cardiac stem/progenitor cells and respond to heart injury.
Bmi-1 high-expressing cells enrich cardiac stem/progenitor cells and respond to heart injury.
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Bmi-1高表达细胞丰富心脏干/祖细胞并对心脏损伤做出反应
DOI：
10.1111/jcmm.13889
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发表时间：
2019-01
影响因子：
5.3
通讯作者：
Xie Y
中科院分区：
文献类型：
--
作者：
Song Y;Zhao M;Xie Y;Zhu T;Liang W;Sun B;Liu W;Wu L;Lu G;Li TS;Yin T;Xie Y
Bmi-1基因被认为是一种癌基因，但最近被证明在组织特异性干细胞的自我更新中发挥作用。通过BMI-1GFP/+小鼠，我们研究了BMI-1在心脏干/祖细胞和心肌修复中的作用。逆转录-聚合酶链式反应和流式细胞仪分析表明，心脏侧−-1的表达明显高于CD45-−-119CD31-−心肌细胞的表达。BMI-1 GFP hi亚群中发现更多的SCA-1+心肌干/祖细胞，这些BMI-1 GFP hi心肌细胞在体外具有向SMM+平滑肌样细胞和TNT+心肌细胞样细胞分化的潜能。沉默BMI-1可显著抑制心肌细胞的增殖和分化。另外，心肌梗死导致BMI-1 GFP在人群中显著增加(2.7倍)，主要是在梗死区和交界区。这些初步数据表明，Bmi-1hi心脏细胞富含心脏干/祖细胞，可能在心肌修复中发挥作用。
Bmi‐1 gene is well recognized as an oncogene, but has been recently demonstrated to play a role in the self‐renewal of tissue‐specific stem cells. By using Bmi‐1GFP /+ mice, we investigated the role of Bmi‐1 in cardiac stem/progenitor cells and myocardial repair. RT‐PCR and flow cytometry analysis indicated that the expression of Bmi‐1 was significantly higher in cardiac side population than the main population from CD45−Ter119− CD31− heart cells. More Sca‐1+ cardiac stem/progenitor cells were found in Bmi‐1 GFP hi subpopulation, and these Bmi‐1 GFP hi heart cells showed the potential of differentiation into SMM + smooth muscle‐like cells and TnT+ cardiomyocyte‐like cells in vitro. The silencing of Bmi‐1 significantly inhibited the proliferation and differentiation of heart cells. Otherwise, myocardial infarction induced a significantly increase (2.7‐folds) of Bmi‐1 GFP hi population, mainly within the infarction and border zones. These preliminary data suggest that Bmi‐1hi heart cells are enriched in cardiac stem/progenitor cells and may play a role in myocardial repair.
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影响因子：
30.8
作者：
Sangiorgi, Eugenio;Capecchi, Mario R.
通讯作者：
Capecchi, Mario R.
影响因子：
11.1
作者：
Malliaras, Konstantinos;Ibrahim, Ahmed;Tseliou, Eleni;Liu, Weixin;Sun, Baiming;Middleton, Ryan C.;Seinfeld, Jeffrey;Wang, Lai;Sharifi, Behrooz G.;Marban, Eduardo
通讯作者：
Marban, Eduardo
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
12.4
作者：
Herrero, Diego;Tome, Maria;Bernad, Antonio
通讯作者：
Bernad, Antonio
BMI1（+）心脏祖细胞在急性损伤后有助于心肌修复。
影响因子：
7.5
作者：
Valiente-Alandi I;Albo-Castellanos C;Herrero D;Sanchez I;Bernad A
通讯作者：
Bernad A