Stimulation of endogenous cardioblasts by exogenous cell therapy after myocardial infarction.
Stimulation of endogenous cardioblasts by exogenous cell therapy after myocardial infarction.
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DOI：
10.1002/emmm.201303626
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发表时间：
2014-06
影响因子：
11.1
通讯作者：
Marban, Eduardo
中科院分区：
文献类型：
--
作者：
Malliaras, Konstantinos;Ibrahim, Ahmed;Tseliou, Eleni;Liu, Weixin;Sun, Baiming;Middleton, Ryan C.;Seinfeld, Jeffrey;Wang, Lai;Sharifi, Behrooz G.;Marban, Eduardo
关键词：
成年哺乳动物内源性心肌细胞祖细胞的身份、起源和生理作用仍存在争议。使用诱导的遗传标记的方法来识别小的非肌细胞表达心脏标记物，我们发现，激活的内源性成心细胞很少在正常成年小鼠心脏明显。然而，心肌梗死导致损伤部位的成心细胞显著活化。基因标记的分离的成心肌细胞表达心脏转录因子和肌节蛋白，表现出自发收缩，并在体内注射到未标记的受体心脏后形成成熟的心肌细胞。活化的成心细胞不是来源于造血接种、心肌细胞去分化或仅仅是预先形成的祖细胞库的扩增。心球源性细胞的细胞疗法增强先天性成心脏细胞介导的组织再生，部分是通过移植细胞分泌基质细胞衍生因子1。因此，外源性细胞刺激内源性成心细胞介导损伤心肌的治疗性再生。
Controversy surrounds the identity, origin, and physiologic role of endogenous cardiomyocyte progenitors in adult mammals. Using an inducible genetic labeling approach to identify small non-myocyte cells expressing cardiac markers, we find that activated endogenous cardioblasts are rarely evident in the normal adult mouse heart. However, myocardial infarction results in significant cardioblast activation at the site of injury. Genetically labeled isolated cardioblasts express cardiac transcription factors and sarcomeric proteins, exhibit spontaneous contractions, and form mature cardiomyocytes in vivo after injection into unlabeled recipient hearts. The activated cardioblasts do not arise from hematogenous seeding, cardiomyocyte dedifferentiation, or mere expansion of a preformed progenitor pool. Cell therapy with cardiosphere-derived cells amplifies innate cardioblast-mediated tissue regeneration, in part through the secretion of stromal cell-derived factor 1 by transplanted cells. Thus, stimulation of endogenous cardioblasts by exogenous cells mediates therapeutic regeneration of injured myocardium.
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影响因子：
20.1
作者：
Bailey B;Fransioli J;Gude NA;Alvarez R Jr;Zhang X;Gustafsson ÅB;Sussman MA
通讯作者：
Sussman MA
影响因子：
64.8
作者：
Alvarez-Dolado, M;Pardal, R;Alvarez-Buylla, A
通讯作者：
Alvarez-Buylla, A
影响因子：
64.5
作者：
Kretzschmar, Kai;Watt, Fiona M.
通讯作者：
Watt, Fiona M.
影响因子：
9.7
作者：
Kanki, Sachiko;Segers, Vincent F. M.;Lee, Richard T.
通讯作者：
Lee, Richard T.
影响因子：
37.8
作者：
Dong, Feng;Harvey, James;Penn, Marc S.
通讯作者：
Penn, Marc S.