Na,K‐ATPase α3 subunit in the goldfish retina during optic nerve regeneration
Na,K‐ATPase α3 subunit in the goldfish retina during optic nerve regeneration
复制标题
视神经再生过程中金鱼视网膜中的Na,K-ATP酶α3亚基
DOI：
10.1046/j.0022-3042.2002.00771.x
复制
发表时间：
2002
影响因子：
4.7
通讯作者：
S. Kato
中科院分区：
文献类型：
--
作者：
Z. W. Liu;T. Matsukawa;K. Arai;M. Devadas;H. Nakashima;M. Tanaka;K. Mawatari;S. Kato
金鱼的视神经损伤后可以再生。为了了解视神经再生的分子机制，我们发现了视神经再生早期表达上调的基因。将金鱼视网膜横断后5 构建的文库与视网膜轴切伤或正常视网膜的cDNADNA探针进行差异杂交。其中1个克隆为␣Na，K-ATPase催化亚基α3亚基。Northern杂交结果显示，视神经切断后2 d表达水平显著升高，5~10 d达高峰，随后逐渐下降，至45 d恢复至对照水平。原位杂交和免疫组织化学染色都揭示了视神经切断后这种一过性视网膜改变的位置。视神经切断后5~20 ，仅在神经节细胞层和视神经纤维层表达增强。在组织块培养系统中，视神经切断后7 天的视网膜神经突起自发生长。低浓度哇巴因(50~100 nm)可完全阻断损伤视网膜的自发突起生长。综上所述，这些数据表明Na，K-ATPaseα3亚单位的上调参与了神经节细胞轴突的再生。
The goldfish optic nerve can regenerate after injury. To understand the molecular mechanism of optic nerve regrowth, we identified genes whose expression is specifically up‐regulated during the early stage of optic nerve regeneration. A cDNA library constructed from goldfish retina 5 days after transection was screened by differential hybridization with cDNA probes derived from axotomized or normal retina. Of six cDNA clones isolated, one clone was identified as the␣Na,K‐ATPase catalytic subunit α3 isoform by high‐ sequence homology. In northern hybridization, the expression level of the mRNA was significantly increased at 2 days and peaked at 5–10 days, and then gradually decreased and returned to control level by 45 days after optic nerve transection. Both in situ hybridization and immunohistochemical staining have revealed the location of this transient retinal change after optic nerve transection. The increased expression was observed only in the ganglion cell layer and optic nerve fiber layer at 5–20 days after optic nerve transection. In an explant culture system, neurite outgrowth from the retina 7 days after optic nerve transection was spontaneously promoted. A low concentration of ouabain (50–100 nm) completely blocked the spontaneous neurite outgrowth from the lesioned retina. Together, these data indicate that up‐regulation of the Na,K‐ATPase α3 subunit is involved in the regrowth of ganglion cell axons after axotomy.
影响因子：
--
作者：
TAKEYASU, K;LEMAS, V;FAMBROUGH, DM
通讯作者：
FAMBROUGH, DM
影响因子：
2.9
作者：
LUTSENKO, S;KAPLAN, JH
通讯作者：
KAPLAN, JH
DOI：
10.1016/s0021-9258(17)39369-9
发表时间：
1985-08
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
M. Roth;N. Tanese;S. Goff
通讯作者：
M. Roth;N. Tanese;S. Goff