Physiological loading of tendons induces scleraxis expression in epitenon fibroblasts.
Physiological loading of tendons induces scleraxis expression in epitenon fibroblasts.
复制标题
DOI：
10.1002/jor.21550
复制
发表时间：
2012-04
影响因子：
2.8
通讯作者：
Brooks, Susan V.
中科院分区：
文献类型：
--
作者：
Mendias, Christopher L.;Gumucio, Jonathan P.;Bakhurin, Konstantin I.;Lynch, Evan B.;Brooks, Susan V.
sccleraxis是一种bHLH转录因子，在肌腱胚胎发育过程中促进成纤维细胞增殖和基质合成发挥核心作用。硬化轴（Scx - / -）靶向失活的小鼠不能正常形成肢体肌腱，但硬化轴在调节成年生物肌腱生长和适应中的作用尚不清楚。为了确定肌腱的生理生长刺激是否会改变硬化轴的表达，我们将在硬化轴启动子（ScxGFP）的控制下表达GFP的成年小鼠进行了为期六周的跑步机训练，旨在诱导跟腱的适应性生长。年龄匹配的久坐ScxGFP小鼠作为对照。在久坐小鼠的外延区很少观察到硬化轴的表达，但在跑步机训练中，硬化轴在成纤维细胞中得到强烈表达，这些成纤维细胞似乎从外延区出现并迁移到肌腱束的表面区域。通过qPCR测量，跑步机训练也导致硬化、肌腱调节蛋白和I型胶原蛋白基因表达的增加。这些结果表明，除了调节肢体肌腱的胚胎形成外，硬化在成年肌腱对生理负荷的适应中也起着重要作用。
Scleraxis is a bHLH transcription factor that plays a central role in promoting fibroblast proliferation and matrix synthesis during the embryonic development of tendons. Mice with a targeted inactivation of scleraxis (Scx−/−) fail to properly form limb tendons, but the role that scleraxis has in regulating the growth and adaptation of tendons of adult organisms is unknown. To determine if scleraxis expression changes in response to a physiological growth stimulus to tendons, we subjected adult mice that express GFP under the control of the scleraxis promoter (ScxGFP) to a six week treadmill training program designed to induce adaptive growth in Achilles tendons. Age matched sedentary ScxGFP mice were used as controls. Scleraxis expression was sparsely observed in the epitenon region of sedentary mice, but in response to treadmill training, scleraxis was robustly expressed in fibroblasts that appeared to be emerging from the epitenon and migrating into the superficial regions of tendon fascicles. Treadmill training also led to an increase in scleraxis, tenomodulin, and type I collagen gene expression as measured by qPCR. These results suggest that in addition to regulating the embryonic formation of limb tendons, scleraxis also appears to play an important role in the adaptation of adult tendons to physiological loading.
登录
查看更多内容
影响因子：
4.8
作者：
Lejard, Veronique;Brideau, Gaelle;Rossert, Jerome
通讯作者：
Rossert, Jerome
影响因子：
4.8
作者：
Lorda-Diez, Carlos I.;Montero, Juan A.;Hurle, Juan M.
通讯作者：
Hurle, Juan M.
影响因子：
4.6
作者：
Pryce, Brian A.;Watson, Spencer S.;Schweitzer, Ronen
通讯作者：
Schweitzer, Ronen
影响因子：
4.6
作者：
Murchison, Nicholas D.;Price, Brian A.;Schweitzer, Ronen
通讯作者：
Schweitzer, Ronen
影响因子：
82.9
作者：
Bi, Yanming;Ehirchiou, Driss;Young, Marian F.
通讯作者：
Young, Marian F.