Molecular characterization, receptor binding property, and replication in chickens and mice of H9N2 avian influenza viruses isolated from chickens, peafowls, and wild birds in eastern China.
Molecular characterization, receptor binding property, and replication in chickens and mice of H9N2 avian influenza viruses isolated from chickens, peafowls, and wild birds in eastern China.
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从中国东部鸡、孔雀和野鸟中分离的 H9N2 禽流感病毒的分子特征、受体结合特性以及在鸡和小鼠中的复制
DOI：
10.1080/22221751.2021.1999778
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发表时间：
2021-12
影响因子：
13.2
通讯作者：
Chen H
中科院分区：
文献类型：
--
作者：
Guo J;Wang Y;Zhao C;Gao X;Zhang Y;Li J;Wang M;Zhang H;Liu W;Wang C;Xia Y;Xu L;He G;Shen J;Sun X;Wang W;Han X;Zhang X;Hou Z;Jin X;Peng N;Li Y;Deng G;Cui P;Zhang Q;Li X;Chen H
H9 N2禽流感病毒在鸟类中广泛流行，由于其在哺乳动物中的毒性和传播性增强，对人类构成越来越大的威胁。积极监察H9 N2病毒在不同禽鸟宿主体内的流行情况及演变，将有助制订根除措施。我们从2017年至2019年中国东部的鸡、绿色孔雀和野生鸟类中分离出16种H9 N2病毒，并对其比较遗传进化、受体结合特异性、抗原多样性、复制和鸡和小鼠传播进行了表征。系统进化分析表明，绿色孔雀病毒和天鹅保护株与我国东部地区流行的禽流感H9 N2病毒具有相同的祖先，而7株野鸟病毒属于野鸟谱系。属于家禽谱系的鸡、孔雀和天鹅H9 N2病毒优先识别α-2，6-连接的唾液酸（类人受体），但野鸟谱系病毒可以类似地结合α-2，3（禽样受体）和类人受体。有趣的是，家禽谱系的H9 N2病毒复制良好且传播效率高，但野鸟谱系的病毒复制和传播效率较低。重要的是，家禽谱系的H9 N2病毒在哺乳动物细胞和小鼠中的复制滴度高于野鸟谱系的病毒。总之，我们的研究表明，H9 N2病毒在家禽、野鸟和观赏鸟中的共同传播增加了它们在不同鸟类中的交叉传播风险，因为它们广泛传播。
H9N2 avian influenza viruses are widely prevalent in birds and pose an increasing threat to humans because of their enhanced virulence and transmissibility in mammals. Active surveillance on the prevalence and evolution of H9N2 viruses in different avian hosts will help develop eradication measures. We isolated 16 H9N2 viruses from chickens, green peafowls, and wild birds in eastern China from 2017 to 2019 and characterized their comparative genetic evolution, receptor-binding specificity, antigenic diversity, replication, and transmission in chickens and mice. The phylogenetic analysis indicated that the green peafowl viruses and swan reassortant shared the same ancestor with the poultry H9N2 viruses prevalent in eastern China, while the seven wild bird viruses belonged to wild bird lineage. The chicken, peafowl, and swan H9N2 viruses that belonged to the poultry lineage preferentially recognized α-2, 6-linked sialic acids (human-like receptor), but the wild bird lineage viruses can bind both α-2, 3 (avian-like receptor) and human-like receptor similarly. Interestingly, the H9N2 viruses of poultry lineage replicated well and transmitted efficiently, but the viruses of wild bird lineage replicated and transmitted with low efficiency. Importantly, the H9N2 viruses of poultry lineage replicated in higher titer in mammal cells and mice than the viruses of wild birds lineage. Altogether, our study indicates that co-circulation of the H9N2 viruses in poultry, wild birds, and ornamental birds increased their cross-transmission risk in different birds because of their widespread dissemination.
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1994-2013年中国不同宿主分离的H9N2流感病毒基因进化
影响因子：
13.2
作者：
Li C;Wang S;Bing G;Carter RA;Wang Z;Wang J;Wang C;Wang L;Wu G;Webster RG;Wang Y;Sun H;Sun Y;Liu J;Pu J
通讯作者：
Pu J
人类感染新型禽源甲型流感 (H7N9) 病毒。
影响因子：
158.5
作者：
Gao, Rongbao;Cao, Bin;Shu, Yuelong
通讯作者：
Shu, Yuelong
影响因子：
9.4
作者：
Butt, KM;Smith, GJD;Guan, Y
通讯作者：
Guan, Y
影响因子：
64.8
作者：
Imai, Masaki;Watanabe, Tokiko;Hatta, Masato;Das, Subash C.;Ozawa, Makoto;Shinya, Kyoko;Zhong, Gongxun;Hanson, Anthony;Katsura, Hiroaki;Watanabe, Shinji;Li, Chengjun;Kawakami, Eiryo;Yamada, Shinya;Kiso, Maki;Suzuki, Yasuo;Maher, Eileen A.;Neumann, Gabriele;Kawaoka, Yoshihiro
通讯作者：
Kawaoka, Yoshihiro
DOI：
10.1126/science.1213362
发表时间：
2012-06-22
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Herfst S;Schrauwen EJ;Linster M;Chutinimitkul S;de Wit E;Munster VJ;Sorrell EM;Bestebroer TM;Burke DF;Smith DJ;Rimmelzwaan GF;Osterhaus AD;Fouchier RA
通讯作者：
Fouchier RA