Rat Dlx5 is expressed in the subventricular zone and promotes neuronal differentiation.
Rat Dlx5 is expressed in the subventricular zone and promotes neuronal differentiation.
复制标题
大鼠 Dlx5 在室下区表达并促进神经元分化。
DOI：
10.1590/s0100-879x2009007500034
复制
发表时间：
2010
期刊：
影响因子：
--
通讯作者：
H. Yang
中科院分区：
文献类型：
--
作者：
H. Shu;F. Gao;C. ‐. Zhang;S. Y. Liu;Z. Zhang;Y. C. Song;K. Qiu;H. Yang
神经前体细胞的分子机制和潜在的临床应用是近年来研究的热点。Dlx 5是一个同源异型盒转录因子，与果蝇的distal-less基因相关，在前脑发育过程中起重要作用。脑室下区（SVZ）在成人大脑窝藏最丰富的神经前体细胞。SVZ前部（SVZa）包含SVZ中最具代表性的神经前体。进一步的研究是必要的，以阐明Dlx 5相关基因如何调节SVZa神经前体细胞的分化。在这里，我们采用免疫组织化学和分子生物学技术研究Dlx 5和相关的同源异型盒基因Er 81和Islet 1在新生大鼠脑和体外培养的SVZa神经前体的表达。我们的研究结果表明，Dlx 5和Er 81也高度表达的SVZa，吻侧迁移流，嗅球。Islet 1仅在纹状体中表达。在培养的SVZa神经前体细胞中，Dlx 5 mRNA的表达随着随后的细胞传代而逐渐降低，并在传代4时完全丧失。我们还转染了Dlx 5重组质粒，发现Dlx 5过表达促进体外培养的SVZa神经前体细胞的神经元分化。综上所述，我们的数据表明，Dlx 5在神经元分化过程中起着重要作用。
The molecular mechanisms and potential clinical applications of neural precursor cells have recently been the subject of intensive study. Dlx5, a homeobox transcription factor related to the distal-less gene in Drosophila, was shown to play an important role during forebrain development. The subventricular zone (SVZ) in the adult brain harbors the largest abundance of neural precursors. The anterior SVZ (SVZa) contains the most representative neural precursors in the SVZ. Further research is necessary to elucidate how Dlx5-related genes regulate the differentiation of SVZa neural precursors. Here, we employed immunohistochemistry and molecular biology techniques to study the expression of Dlx5 and related homeobox genes Er81 and Islet1 in neonatal rat brain and in in vitro cultured SVZa neural precursors. Our results show that Dlx5 and Er81 are also highly expressed in the SVZa, rostral migratory stream, and olfactory bulb. Islet1 is only expressed in the striatum. In cultured SVZa neural precursors, Dlx5 mRNA expression gradually decreased with subsequent cell passages and was completely lost by passage four. We also transfected a Dlx5 recombinant plasmid and found that Dlx5 overexpression promoted neuronal differentiation of in vitro cultured SVZa neural precursors. Taken together, our data suggest that Dlx5 plays an important role during neuronal differentiation.
影响因子：
4.6
作者：
H. Wichterle;D. Turnbull;S. Nery;G. Fishell;A. Álvarez-Buylla
通讯作者：
H. Wichterle;D. Turnbull;S. Nery;G. Fishell;A. Álvarez-Buylla
Distal-less/Dlx 同源盒基因的发育功能。
DOI：
10.1242/dev.129.19.4371
发表时间：
2002
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Panganiban,Grace;Rubenstein,JohnLR
通讯作者：
Rubenstein,JohnLR