Microfluidic devices for fluidic circulation and mixing improve hybridization signal intensity on DNA arrays.
Microfluidic devices for fluidic circulation and mixing improve hybridization signal intensity on DNA arrays.
复制标题
用于流体循环和混合的微流体装置可提高 DNA 阵列上的杂交信号强度。
作者：
P. Yuen;Guangshan Li;Y. Bao;U. Muller
生物分子与显微镜载玻片上表面安装材料的反应通常受到缓慢扩散动力学的限制，特别是在扩散是唯一混合方式的小体积情况下。对于只有少量分析物可用并且所需的反应体积限制了分析物浓度的反应来说，这是一个特别的问题。开发了一种由两个互连的 9 mm x 37.5 mm 反应室组成的低容量微流体装置，以允许在显微镜载玻片的大部分表面上进行混合和闭环流体循环。通过标准磁力搅拌器驱动的磁力搅拌棒的旋转，将流体样品从一个反应室移至另一个反应室。我们证明，这种闭环装置可以有效地实现不同试剂的循环和混合，溶液的粘度在 1 到 16.2 厘泊之间变化。我们还通过微阵列杂交的例子表明，在扩散速率受到限制的条件下，通过流体混合可以将反应效率提高 2-5 倍。为了进行比较，使用仅覆盖闭环装置一半反应区域的一次性商用装置获得了类似的结果。
Reactions of biomolecules with surface mounted materials on microscope slides are often limited by slow diffusion kinetics, especially in low volumes where diffusion is the only means of mixing. This is a particular problem for reactions where only small amounts of analyte are available and the required reaction volume limits the analyte concentration. A low volume microfluidic device consisting of two interconnected 9 mm x 37.5 mm reaction chambers was developed to allow mixing and closed loop fluidic circulation over most of the surface of a microscope slide. Fluid samples are moved from one reaction chamber to the other by the rotation of a magnetic stirring bar that is driven by a standard magnetic stirrer. We demonstrate that circulation and mixing of different reagents can be efficiently accomplished by this closed loop device with solutions varying in viscosity from 1 to 16.2 centipoise. We also show by example of a microarray hybridization that the reaction efficiency can be enhanced 2-5 fold through fluid mixing under conditions where diffusion is rate limiting. For comparison, similar results were achieved with a disposable commercial device that covers only half of the reaction area of the closed loop device.
影响因子：
2.7
作者：
Hegde, P;Qi, R;Quackenbush, J
通讯作者：
Quackenbush, J