Molecular imaging with engineered physiology.
Molecular imaging with engineered physiology.
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DOI：
10.1038/ncomms13607
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发表时间：
2016-12-02
影响因子：
16.6
通讯作者：
Jasanoff, Alan
中科院分区：
文献类型：
--
作者：
Desai, Mitul;Slusarczyk, Adrian L.;Chapin, Ashley;Barch, Mariya;Jasanoff, Alan
体内成像技术是评价生物系统的有力工具。然而，由于现有的光学、磁性和放射性成像探针机制的限制，将图像信号与精确的分子现象相关联可能具有挑战性。在这里，我们证明了一个概念，分子成像，绕过传统的成像剂的需要，通过扰动的内源性多模态对比提供的脉管系统。降钙素基因相关肽的变体人工激活大鼠脑中的血管舒张通路，并诱导容易通过光学和磁共振成像测量的对比度变化。基于CGRP的药剂在深部组织中以纳摩尔浓度诱导效应，并且可以被工程化为可转换的分析物依赖性形式和适于分子成像或细胞追踪的遗传编码的报告物。因此，这种人工设计的生理变化为活生物体中分子事件的灵敏分析提供了高度通用的手段。 脉管系统在磁共振成像（MRI）中产生强的内源性对比。Desai等人在本文中报告了源自血管扩张剂降钙素基因相关肽的遗传编码成像探针，其允许通过光学成像或MRI中的血液动力学变化来可视化分子事件。
In vivo imaging techniques are powerful tools for evaluating biological systems. Relating image signals to precise molecular phenomena can be challenging, however, due to limitations of the existing optical, magnetic and radioactive imaging probe mechanisms. Here we demonstrate a concept for molecular imaging which bypasses the need for conventional imaging agents by perturbing the endogenous multimodal contrast provided by the vasculature. Variants of the calcitonin gene-related peptide artificially activate vasodilation pathways in rat brain and induce contrast changes that are readily measured by optical and magnetic resonance imaging. CGRP-based agents induce effects at nanomolar concentrations in deep tissue and can be engineered into switchable analyte-dependent forms and genetically encoded reporters suitable for molecular imaging or cell tracking. Such artificially engineered physiological changes, therefore, provide a highly versatile means for sensitive analysis of molecular events in living organisms. The vasculature produces strong endogenous contrast in magnetic resonance imaging (MRI). Here Desai et al. report genetically encoded imaging probes derived from the vasodilator, calcitonin gene-related peptide, which allows visualization of molecular events via haemodynamic changes in optical imaging or MRI.
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